Effects of High Sugar Diet and Condensed Tannin Supplementation on Growth Performance,Body Composition,Serum Metabolites,Intestinal Short-Chain Fatty Acid Contents and Hormone Gene Expression of Sea Bass(Lateolabrax maculatus)
This study was conducted to investigate the effects of high sugar diet and condensed tannin(CT)supplementation on growth performance,body composition,serum metabolites,intestinal short-chain fatty acid contents and hormone gene expression of sea bass(Lateolabrax maculatus).A total of 480 healthy sea bass with an initial body weight of(10.85±0.02)g were randomly divided into 3 groups with 4 replicates per group and 40 fish per replicate.Sea bass in the three groups were fed conventional diet(containing 20.0％flour,G1 group),high sugar diet(containing 45.8％flour,G2 group)and high sugar diet supplemented with CT(con-taining 45.8％flour and 0.1％CT,G3 group),respectively.The experiment lasted for 56 days.The results showed as follows:1)compared with G1 group,the final body weight,weight gain rate and specific growth rate of sea bass in G2 group were significantly decreased(P＜0.05),and the feed coefficient,condition fac-tor,hepatosomatic index and viscerosomatic index were significantly increased(P＜0.05).Compared with G2 group,the final body weight,weight gain rate and specific growth rate in G3 group were significantly in-creased(P＜0.05),and the feed coefficient was significantly decreased(P＜0.05).2)Compared with Gl group,the contents of moisture,crude protein and crude ash in the whole body of sea bass in G2 and G3 groups were significantly decreased(P＜0.05).3)At 1 h after feeding,the serum glucose content of sea bass in G2 group was significantly higher than that in G1 and G3 groups(P＜0.05);at 3 and 6 h after feeding,there was no significant difference in serum glucose content among all groups(P＞0.05).The serum glutamic oxaloacetic transaminase activity and high-density lipoprotein cholesterol content of sea bass in G2 group were significantly higher than those in G1 and G3 groups(P＜0.05),but there were no significant differences in the above indices between G1 and G3 groups(P＞0.05).4)The contents of acetic acid,butyric acid and total short-chain fatty acid in intestine of sea bass in all groups were in the order of G1 group＞G3 group＞G2 group from high to low,and the differences among groups were all significant(P＜0.05).5)The mRNA relative ex-pression levels of glucagon-like peptide-1(GLP-1),glucagon-like peptide-2(GLP-2),sodium-dependent glucose cotransporter 1(SGLT1),sodium-dependent glucose cotransporter 2(SGLT2)and glucose transporter 2(GLUT2)in intestine of sea bass in G2 group were significantly lower than those in G1 and G1 groups(P＜0.05),the YY peptide(PYY)mRNA relative expression level in intestine in G2 group was significantly high-er than that in G1 and G3 groups(P＜0.05),but there were no significant differences between G1 and G3 groups(P＞0.05).In conclusion,the high sugar diet can increase the serum glucose content,induce the liver injury,reduce the intestinal short-chain fatty acid content and expression of glucagon-like peptide and glucose transporter genes,and inhibit the growth and nutrient deposition of sea bass.The supplementation of 0.1％CT in the high sugar diet can repair the liver injury,reduce the serum glucose content by regulating the gene ex-pression of intestinal hormone,and improve the growth performance of sea bass.[Chinese Journal of Animal Nutrition,2024,36(8):5254-5268]
high sugar dietcondensed tanningrowth performanceglucose metabolismLateolabrax macu-latus
万方数据
维普
