Effects of Pediococcus pentosaceus on Growth Performance,Antioxidant Capacity,Intestinal Microbes and Their Metabolites of Juvenile Larimichthys crocea
This study was conducted to investigate the effects of Pediococcus pentosaceus on growth perform-ance,antioxidant capacity,intestinal microbes and their metabolites of juvenile Larimichthys crocea,and to provide reference for the utilization of green feed additives for juvenile Larimichthys crocea.A total of 270 large yellow croaker with an initial body weight of(9.53±0.54)g were randomly divided into 3 groups with 3 cages(replicates)in each group and 30 fish in each cage.The concentrations of Pediococcus pentosaceus in diets of the three groups were 0(PP0 group),107(PP7 group)and 108 CFU/g(PP8 group),respectively,and the culture cycle was 56 days.The results showed as follows:1)compared with the PP0 group,the weight gain rate and specific growth rate in PP7 and PP8 groups were increased(P＞0.05),and the viscerosomatic in-dex was significantly increased(P＜0.05);2)compared with the PP0 group,the moisture content of muscle in PP7 and PP8 groups was significantly increased(P＜0.05),and the crude lipid content of muscle was signif-icantly decreased(P＜0.05);3)compared with the PP0 group,the intestinal crypt depth in PP7 and PP8 groups was significantly decreased(P＜0.05),and the ratio of villus length/crypt depth was significantly in-creased(P＜0.05);4)compared with the PP0 group,the liver catalase(CAT)activity in PP7 group was sig-nificantly increased(P＜0.05),the liver alkaline phosphatase(AKP)activity in PP8 group was significantly increased(P＜0.05),the serum AKP activity in PP7 and PP8 groups was significantly increased(P＜0.05),and the serum lysozyme(LZM)activity in PP7 group was significantly increased(P＜0.05);5)compared with the PP0 group,the PD_whole_tree index in PP7 group was significantly increased(P＜0.05),the relative abundance of beneficial bacteria Bdellovibrio was significantly increased(P＜0.05),and the relative abundance of harmful bacteria Nocardioides was significantly decreased(P＜0.05);6)compared with the PP0 group,the concentrations of asparagine-lysine(Asn-Lys)and temsirolimus in PP7 group were significantly increased(P＜0.05),while the concentrations of urocortisol and galantamine were significantly decreased(P＜0.05).In summary,Pediococcus pentosaceus has the ability to regulate the metabolism of intestinal microbes,optimize the intestinal microecology by improving the structure of intestinal microbial community structure and intestinal microstructure,improve the digestive and absorption function of juvenile Larimichthys crocea,and enhance its antioxidant capacity and immunity.[Chinese Journal of Animal Nutrition,2024,36(8):5283-5296]
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