Curcumin Improves Adverse Effects of Oxidized Fish Oil on Intestine of Pelteobagrus fulvidraco
This study was conducted to investigate the effects of curcumin on the intestinal digestive enzyme ac-tivities,tissue morphology and microflora structure of Pelteobagrus fulvidraco fed diets containing oxidized fish oil.Six isoproteic and isolipidic diets were prepared which contained 30％fresh fish oil(T1 group),30％oxi-dised fish oil(T2 group),30％oxidized fish oil+0.02％curcumin(T3 group),30％oxidized fish oil+0.04％curcumin(T4 group),30％oxidized fish oil+0.06％curcumin(T5 group)and 30％oxidized fish oil+0.08％curcumin(T6 group).A total of 720 healthy Pelteobagrus fulvidraco with an initial body weight of(19.6±0.3)g were randomly distributed into six groups(four replicates in each group and 30 fish in each rep-licate),which were fed with each of the 6 experimental diets.The feeding trial was lasted for 56 days.The re-sults showed as follows:compared with the T1 group,there was no significant difference in intestinal digestive enzyme activities in the T2 group(P＞0.05),but the length of intestinal villi was significantly reduced(P＜0.05);at the genus level,the relative abundances of Pseudomonas and Ralstonia were significantly decreased(P＜0.05),and the relative abundance of Cetobacterium was significantly increased in the T2 group(P＜0.05).Compared with the T2 group,the intestinal trypsin activity,villus width,goblet cell number were sig-nificantly increased in the T6 group(P＜0.05),the intestinal lipase activity was significantly increased in the T4 to T6 groups(P＜0.05),the intestinal villus length was significantly increased in the T3 to T6 groups(P＜0.05),and the intestinal muscular thickness was significantly increased in the T3 and T6 groups(P＜0.05);at the genus level,the relative abundances of Clostridium and Candidatus_Arthromitus were significantly increased in the T4 group(P＜0.05),the relative abundance of Pseudomonas was significantly increased(P＜0.05)and the relative abundance of Cetobacterium was significantly decreased(P＜0.05)in the T4 to T6 groups,the rel-ative abundance of Ralstonia was significantly increased in the T5 and T6 groups(P＜0.05).As dietary curcu-min supplemental levels increasing,the intestinal lipase activity showed a linear effect(P=0.001),the villi length showed a quadratic effect(P＜0.001),the villi width showed a linear effect(P=0.001)and a quadrat-ic effect(P＜0.001),and the goblet cell number showed a linear effect(P=0.030)and a quadratic effect(P=0.007);at genus level,the relative abundance of Pseudomonas showed a linear effect(P＜0.001)and a quadratic effect(P＜0.001),the relative abundance of Cetobacterium showed a linear effect(P=0.001)and a quadratic effect(P=0.001),and the relative abundance of Ralstonia showed a quadratic effect(P＜0.001).In conclusion,adding 3％oxidized fish oil in the Pelteobagrus fulvidraco diet induces intestinal tissue structure damage,and adding 0.04％to 0.08％of curcumin can increase the intestinal trypsin and lipase activities as well as the relative abundances of beneficial bacteria Pseudomonas and Candidatus_Arthromitus,and repair the structural damage to intestinal villius of Pelteobagrus fulvidraco induced by oxidized fish oil.[Chinese Journal of Animal Nutrition,2024,36(8):5297-5307]
oxidized fish oilcurcuminPelteobagrus fulvidracointestinal health
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