Alleviating Effect of Bamboo Leaf Flavonoids on Oxidative Damage in Bovine Mammary Epithelial Cells and Its Effect on Milk Fat Synthesis
The purpose of this study was to explore the alleviating effect of bamboo leaf flavonoids(BLF)on oxidative damage in bovine mammary epithelial cells(BMECs)and its effect on milk fat synthesis.With BMECs as study subjects,the following operations were carried out:cells were seeded in maintenance medium without any substance for 24 h to set the control group 1;cells were seeded in maintenance medium containing 800 μmol/L hydrogen peroxide(H2O2)for 8 h to set the injury group;cells were seeded in maintenance me-dium containing 80 μg/mL BLF for 24 h to set the control group 2;cells were seeded in maintenance medium containing 80 μg/mL BLF for 24 h,then replaced in maintenance medium containing 800 µmol/L H2O2 for 8 h to set the protection group.The antioxidant indexes in cells were examined using the kit method,the mR-NA relative expression levels of antioxidative,apoptosis and milk fat-related genes were measured by real-time fluorescence quantification PCR,and the expression levels of milk fat-related proteins were measured by West-em blotting.The results showed as follows:1)compared with the injury group,the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)in cells of the protection group were significantly increased(P＜0.05),and the malondialdehyde(MDA)content was significantly decreased(P＜0.05).2)Compared with the control group 1,the content of reactive oxygen species(ROS)in cells of the injured group was significantly increased(P＜0.05),and BLF pretreatment could significantly inhibit ROS production in oxidative damaged cells(P＜0.05).3)Compared with the injured group,the mRNA relative ex-pression levels of apoptotic genes B cell lymphoma/leukemia-2-related X protein(Bax)and cysteine aspartate protease 3(Caspase3)in cells of the protection group were significantly decreased(P＜0.05),the mRNA rel-ative expression levels of anti-apoptotic gene B lymphocytoma 2(Bcl2)was significantly increased(P＜0.05),and the mRNA relative expression levels of antioxidant genes heme oxygenase-1(HO-1),quinone ox-idoreductase 1(NQO1)and nuclear transcription factor E2-associated factor 2(Nrf2)were significantly in-creased(P＜0.05).4)Compared with the control group 1,the lipid droplets(LDs)content decreased in the injured group,the LDs content increased in the protection group,and the triglycerides(TGs)content did not return to normal level but was significantly different compared with the injured group(P＜0.05).5)Compared with the injured group,the mRNA relative expression levels of acetyl-CoA carboxylase α(ACACA),acetyl-CoA synthetase 2(ACSS2),fatty acid-binding protein 3(FABP3),fatty acid synthetase(FASN),peroxi-some proliferator-activated receptor γ(PPARγ),cholesterol regulatory element binding protein 1(SREBP1)and stearoyl-CoA desaturase(SCD)in cells of the protection group were significantly increased(P＜0.05).The relative expression levels of milk fat synthesis related proteins ACSS2,FABP3,PPARγ and SCD in cells were all significantly increased of the protection group compared with the control group 1(P＜0.05).To sum up,BLF can not only by promoting the expression of Nrf2 and its downstream antioxidant genes,thus enhance the antioxidant capacity of BMECs,reduce the oxidative damage caused by oxidative stress,and BLF can also effectively improve the expression of milk fat related genes and proteins,thus promote the synthesis of milk fat,effectively reduce the effects of oxidative damage on the body milk fat synthesis.[Chinese Journal of Ani-mal Nutrition,2024,36(8):5340-5352]
万方数据
维普
