Regulatory Effects of Terpinen-4-ol on Liver Development,Antioxidant Capacity and Immune Function of Weaned Piglets under Immune Stress Induced by Lipopolysaccharide
The aim of this study was to investigate the regulatory effects of terpinen-4-ol(TER)on liver de-velopment,antioxidant capacity and immune function of weaned piglets under immune stress induced by LPS.Forty 28-day-old weaned piglets(weaned at 21 days of age)with similar body weight were selected and ran-domly divided into five groups with eight piglets per group.Piglets in the control group(CON group)and li-popolysaccharide group(LPS group)were fed a basal diet,and others in low,medium and high-dose terpin-en-4-ol groups were fed the basal diet supplemented with 30(PLT group),60(PMT group)and 90 mg/kg(PHT group)terpinen-4-ol,respectively.The pre-experimental period lasted for 3 days,and the experimental period lasted for 21 days.At the end of the experiment,the weaned piglets in LPS group and terpinen-4-ol groups were intraperitoneally injected with 100 μg/kg BW of LPS,while others in the control group were in-traperitoneally injected with equivalent dose of 0.9％saline.The results showed as follows:1)compared with CON group,the liver weight and liver index of LPS group were significantly decreased(P＜0.05).Compared with LPS group,the liver weight of PHT group was significantly increased(P＜0.05).2)The cytoplasm of liver cells of CON group was intact,no obvious inflammation and necrosis were found;the liver tissue of LPS group was seriously damaged,including liver cell necrosis,cell swelling,cell membrane structure destruction and nuclear contraction.Compared with CON group,the area and particle size of liver cells of LPS group were significantly decreased(P＜0.05).Compared with LPS group,the area and particle size of liver cells of PLT,PMT and PHT groups were significantly increased(P＜0.05).3)Compared with CON group,the activities of catalase(CAT)and glutathione peroxidase(GSH-Px)in liver of LPS group were significantly decreased(P＜0.05).Compared with LPS group,the liver GSH-Px activity of PLT,PMT and PHT groups was significantly increased(P＜0.05).4)Compared with CON group,the liver interleukin-1β(IL-1β)content of LPS group was significantly increased(P＜0.05).Compared with LPS group,the liver IL-1β content of PMT and PHT groups was significantly decreased(P＜0.05),and the contents of interleukin-6(IL-6)and interleukin-8(IL-8)in liver of PHT group were significantly decreased(P＜0.05).In conclusion,the LPS stimulation can lead to liver damage,liver antioxidant and immune function impairment.Dietary terpinen-4-ol can enhance antioxi-dant capacity and immune function and alleviate liver inflammatory damage of weaned piglets by increasing liv-er antioxidant oxidase activity,reducing pro-inflammatory factor content and increasing anti-inflammatory fac-tor content.[Chinese Journal of Animal Nutrition,2024,36(9):5608-5616]
万方数据
维普
