摘要
本试验旨在探究早期断奶腹泻仔猪血清游离氨基酸谱及其与肠道微生物的相关性.试验选用28日龄断奶的金华猪仔猪,于断奶后7 d分别选择5头健康仔猪(健康组)和5头腹泻仔猪(腹泻组,D组),采集血清和结肠内容物用于血清游离氨基酸含量测定和16S rRNA测序分析.结果表明:1)与健康仔猪相比,腹泻仔猪血清游离氨基酸中苏氨酸、色氨酸、丙氨酸、脯氨酸、丝氨酸、酪氨酸和谷氨酰胺含量显著降低(P＜0.05);血清氨基酸衍生物和代谢产物中牛磺酸、瓜氨酸、鸟氨酸、β-氨基异丁酸、肌肽、羟脯氨酸、精氨基琥珀酸和β-丙氨酸含量显著降低(P＜0.05),血清1-甲基组氨酸含量显著提高(P＜0.05).2)与健康仔猪相比,腹泻仔猪结肠微生物扩增子序列变体(ASV)数量、Chao1指数和Shannon指数显著降低(P＜0.05),Simpson指数显著提高(P＜0.05).主坐标分析(PCoA)显示2组结肠微生物群落呈现不同的聚类.与健康仔猪相比,在门水平上,腹泻仔猪结肠厚壁菌门(Firmicutes)相对丰度显著降低(P＜0.05);在属水平上,腹泻仔猪结肠狭义梭菌属1(Clostridium_sensu_stricto_1)、Lachnoclostridium和土孢杆菌属(Terrisporobacter)相对丰度显著降低(P＜0.05),结肠梭杆菌属(Fusobacterium)相对丰度显著提高(P＜0.05).通过线性判别分析效应大小(LEfSe)分析发现,Lachnoclostridium、土孢杆菌属、瘤胃球菌属(Ruminococcus)、梭杆菌属和拟杆菌属等为2组差异菌属.3)Spearman相关性分析发现,结肠Lachnoclostridium和土孢杆菌属相对丰度均与血清β-丙氨酸、丝氨酸和脯氨酸含量呈显著正相关(P＜0.05),结肠瘤胃球菌属和厌氧棍状菌属(Anaerotruncus)相对丰度均与血清丝氨酸、脯氨酸、瓜氨酸、羟脯氨酸和β-氨基异丁酸含量呈显著正相关(P＜0.05);而结肠梭杆菌属相对丰度与血清丝氨酸、瓜氨酸、羟脯氨酸和β-氨基异丁酸含量氨基酸含量呈显著负相关(P＜0.05),结肠拟杆菌属相对丰度均与血清色氨酸和酪氨酸含量呈显著负相关(P＜0.05).综上可知,早期断奶健康与腹泻仔猪血清游离氨基酸谱和肠道微生物存在差异,且二者存在一定相关性.
Abstract
This experiment was conducted to explore the serum free amino acid profiles and their correlation with intestinal microbiota in early weaned diarrhea piglets.Twenty eight-day-old weaned Jinhua piglets were selected,and each five healthy piglets(healthy group)and five diarrhea piglets(diarrhea group)were select-ed 7 days after weaning.Then the serum and colon contents of piglets were collected for serum free amino acid content determination and 16S rRNA sequencing analysis.The results showed as follows:1)compared with healthy piglets,the contents of threonine,tryptophan,alanine,proline,serine,tyrosine and glutamine in ser-um free amino acids of diarrhea piglets were significantly decreased(P＜0.05);among serum amino acid de-rivatives and metabolites,the contents of taurine,citrulline,ornithine,β-aminoisobutyric acid,carnosine,hydroxyproline,argininosuccinic acid and β-alanine were significantly decreased(P＜0.05),while the 1-meth-ylhistidine content was significantly increased(P＜0.05).2)Compared with healthy piglets,the amplicon se-quence variant(ASV)number,Chao1 index and Shannon index of colonic microbiota of diarrhea piglets sig-nificantly decreased(P＜0.05),while the Simpson index was significantly increased(P＜0.05).Principal co-ordinate analysis(PCoA)showed that the colonic microbiota had different clustering in two groups.Compared with healthy piglets,at the phylum level,the Firmicutes relative abundance in colon of diarrhea piglets was sig-nificantly decreased(P＜0.05);at the genus level,the relative abundances of Clostridium_sensu_stricto_1,Lachnoclostridium and Terrisporobacter in colon of diarrhea piglets were significantly decreased(P＜0.05),while the Fusobacterium relative abundance in colon was significantly increased(P＜0.05).Linear discriminant analysis effect size(LEfSe)analysis showed that Lachnoclostridium,Terrisporobacter,Ruminococcus,Fuso-bacterium and Bacteroidetes were differential genera between two groups.3)Spearman correlation analysis found that the abundances of Lachnoclostridium and Terrisporobacter in colon were significantly positively cor-related with the contents of β-alanine,serine and proline in serum(P＜0.05),and the abundances of Rumino-coccus and Anaerotruncus in colon were significantly positively correlated with the contents of serine,proline,citrulline,hydroxyproline and β-aminoisobutyric acid in serum(P＜0.05);while the Fusobacterium relative a-bundance in colon was significantly negatively correlated the contents of serine,citrulline,hydroxyproline and β-aminoisobutyric acid in serum(P＜0.05),and the Bacteroides relative abundance in colon was significantly negatively correlated with the contents of tryptophan and tyrosine in serum(P＜0.05).In conclusion,there are differences in serum free amino acid profiles and intestinal microbiota between early weaned healthy and diar-rhea piglets,and there is a certain correlation between them.[Chinese Journal of Animal Nutrition,2024,36(9):5617-5629]
维普
万方数据