Resveratrol-Activated Peroxisome Proliferator-Activated Receptor-Gamma Coactivator-1a Alleviates Lipopolysaccharide-Induced Inflammatory Injury and Apoptosis in Bovine Mammary Epithelial Cells
In order to reveal whether resveratrol(RES)alleviated inflammatory reaction of bovine mammary epithelial cells(bMECs)by mediating peroxisome proliferator-activated receptor-gamma coactivator-1α (PGC-1α),this present experiment was performed on the PGC-1α was interfered(si-PGC-1α)by using a one-way randomized trial design method,setting up si-NC+LPS group(hunger medium prepared with 10 µg/mL LPS),si-PGC-1α+LPS group(si-PGC-1α interference fragment+hunger medium prepared with 10 μg/mL LPS),si-NC+RES+LPS group(hunger medium co-prepared with 10 μg/mL LPS and 15 µmol/L RES)and si-PGC-lα+RES+LPS group(si-PGC-1α interference fragment+hunger medium co-prepared with 10 µg/mL LPS and 15 µmol/L RES).The results showed that the addition of LPS after si-PGC-1α significantly up-regu-lated the mRNA relative expression levels of contents of the inflammatory factors interleukin-6(IL-6)and in-terleukin-8(IL-8)(P＜0.05 or P＜0.01),significantly up-regulated the mRNA relative expression level of the oxidizing factor malondialdehyde(MDA)(P＜0.01),significantly up-regulated the mRNA relative expression level of the pro-apoptotic factor B-celllymphoma-2 associated X protein(BAX)and bMECs apoptosis rate(P＜0.05 or P＜0.01),and significantly down-regulated the mRNA relative expression level of the anti-oxidant gene glutathione peroxidase(GSH-Px)(P＜0.01);and RES could reversed this response.When si-PGC-1α was followed by RES treatment,it failed to alleviate the oxidative stress,inflammatory stress and apoptosis produced by LPS-induced bMECs,suggesting that RES played a role in alleviating LPS-induced inflammatory injury and apoptosis in bMECs by activating the PGC-1α.In conclusion,exogenous addition of RES can allevi-ate LPS-induced mitochondrial damage,oxidative stress,inflammatory response and inhibit apoptosis of bMECs by activating the PGC-1α.[Chinese Journal of Animal Nutrition,2024,36(9):5970-5980]
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