Acute Toxicity Test,Sub-Chronic Toxicity Test and Mutagenicity Test of Poly β-Carotene Polymer
This study aimed to evaluate the safety of poly β-carotene polymer through acute toxicity test in mice,sub-chronic toxicity test in rats and mutagenicity test in mice.Acute toxicity test:50 Kunming(KM)mice were randomly divided into 5 groups with 10 mice in each group.The median lethal dose(LD5)0)and 95％confidence interval of poly β-carotene polymer were determined.Sub-chronic toxicity test:80 specific pathogen free(SPF)grade SD rats were randomly divided into 4 groups with 20 rats in each group.The low-,medium-and high-dose groups were gavaged with 100,250 and 500 mg/kg BW poly β-carotene polymer,re-spectively,and the control group was gavaged with an equal volume of vegetable oil for 30 d.The growth per-formance,blood physiological indices,serum biochemical indices,organ indices and histopathology of the rats were determined.Mutagenicity test:50 KM mice were randomly divided into 5 groups with 10 mice in each group.Based on the LD50 of poly β-carotene polymer derived from this test,the low-,middle-and high-dose groups were gavaged with 282.5,565.0 and 1 130.0 mg/kg BW poly β-carotene polymer,respectively;the negative control group was gavaged with an equal volume of vegetable oil,and the positive control group was injected intraperitoneally with 0.4 mL/kg cyclophosphamide.The polychromatic erythrocyte/erythrocyte ratio and polychromatic erythrocyte micronucleus rate were determined.The results showed as follows:1)in the a-cute toxicity test,the LD50 of poly β-carotene polymer was 2 260 mg/kg BW,with a 95％confidence interval of 2 067 to 2 470 mg/kg BW.2)In the sub-chronic toxicity test,the rat's mental appetite and other condi-tions during the test period were good.Compared with the control group,the final weight,average daily gain,average daily feed intake of male rats in the low-,medium-and high-dose groups and female rats in the high dose group were significantly increased(P＜0.01),and the feed to gain ratio was significantly decreased(P＜0.01).The hematocrit of male rats in the medium-dose group was significantly lower than that in the low-dose group(P＜0.05),and there were no significant differences in other blood physiological and serum biochemical indices of rats among all groups(P＞0.05).The kidney index of female rats in middle-and high-dose groups was significantly lower than that in control and low-dose groups(P＜0.05),and there was no significant differ-ence in organ weight and other organ indices of rats among all groups(P＞0.05).Histopathological examina-tion showed that there were no obvious pathological changes in the heart,liver,spleen,kidney and lungs of rats among all groups.3)In the mutagenicity test,compared with the negative control group,the polychromat-ic erythrocyte micronucleus rate of male and female rats in the positive control group was significant increased(P＜0.01),and the polychromatic erythrocyte micronucleus rate of male and female rats in low-,medium-and high-dose groups were no significant difference(P＞0.05).In conclusion,the poly β-carotene polymer has no obvious acute toxicity,sub-chronic toxicity and mutagenicity.The poly β-carotene polymer can improve the growth performance of rats,and can be further developed and utilized as a safe feed additive.[Chinese Journal of Animal Nutrition,2024,36(9):5994-6006]
万方数据
维普
