Effects of Vitamin and Probiotic Composite Additive on Semen Quality and Testicular Function Remodeling of Breeding Roosters
This experiment was conducted to investigate the effects of vitamin and probiotic composite additive on the semen quality and testicular function remodeling of breeding roosters.Fifty healthy 350-day-old female Guifei breeding roosters with similar body weight[(1.49±0.24)kg]were selected and randomly divided into two groups after fasting for 15 days,and each group had 25 roosters.Roosters in control group were fed a basal diet during the recovery period,and roosters in experimental group were fed the basal diet 200 mg/kg vitamin and probiotic com-posite additive during the recovery period.The experiment was divided into pre-feeding period for 7 days,fasting period for 15 days and recovery period for 32 days,for a total of 54 days.The body weight of the two groups at the day before fasting(F0),15 days after fasting(F15)and 32 days after resuming feeding(R32),as well as testicu-lar weight,sperm density and sperm motile at R32 were recorded,and high throughput transcriptomic sequencing(RNA-seq)was performed on the testicular tissue at R32.The results showed as follows:1)at the R32,the body weight of experimental group was significantly higher than that of control group(P＞0.05);the sperm motility of experimental group was significantly higher than that of control group(P＜0.05),but the testicular weight and sperm density were not significantly different between experimental group and control group(P＞0.05).2)RNA-seq analysis was performed on the testis tissues of the two groups,and 57 differential genes were obtained,of which 9 genes were up-regulated and 48 genes were down-regulated.The differential genes were mainly annotated to transmembrane receptor protein tyrosine kinase activator activity,JAK pathway signal transduction adaptor activ-ity,seminal vesicle development,actin filament bundle assembly,and so on.It is mainly concentrated in influenza A,base excision repair,MAPK signaling pathway and intestinal immune network for immunoglobulin A produc-tion pathways.In conclusion,the vitamin and probiotic composite additive can improve semen quality by accelera-ting weight recovery and enhancing sperm motility.Through RNA-seq analysis of testicular tissue,T-cell acute lymphocytic leukemia(TAL1),steroidogenic acute regulatory protein(STAR),late blight resistance gene(BLB1),angiopoietin 4(ANGPT4),Nei endonuclease Ⅷ-like protein 3(NEIL3)and other genes are selected as candidate genes for the study of semen quality and testicular function of breeding roosters.[Chinese Journal of An-imal Nutrition,2024,36(11):7028-7039]
vitamin and probiotic composite additivetestistranscriptomedifferential gene
万方数据
维普
