Effects of Co-Inoculation of Cellulose Decomposition Bacteria and Homogenous Lactic Acid Bacteria on Fermentation Quality,Aerobic Stability,Enzyme Activity and Ruminal Degradability of Silage Maize
The aim of this study was to investigate the effects of co-inoculation of cellulose decomposition bac-teria(CDB)and homogeneous lactic acid bacteria on fermentation quality,aerobic stability,enzyme activity and rumen degradability of silage maize.The whole silage maize was prepared by vacuum bag method,and de-sign three experimental treatments,CK treatment was added only deionized water;LP treatment was mixed in-oculate Lactiplantibacillus plantarum and Pediococcus acidilactici(1∶1,mass ratio),with a total inoculation amount of 5×105 CFU/g FM;TX treatment was inoculated CDB on the basis of LP treatment,the ratio of As-pergillus Niger,Trichoderma viride and Bacillus subtilis in CDB was 2∶1∶1(mass ratio),and the additive a-mount was 0.3％.At the end of fermentation,the nutritional quality,main microbial quantity,aerobic stabili-ty,enzyme activity and rumen degradability of silage maize were analyzed.The results showed as follows:1)the pH,lactic acid(LA)and starch contents and mold quantity of TX treatment were significantly higher than those of CK and LP treatments(P＜0.05).The acetic acid(AA)content of LP treatment was significantly lower than that of CK and TX treatments(P＜0.05).The dry matter(DM)content of CK treatment was sig-nificantly lower than that of LP and TX treatments(P＜0.05).The neutral detergent fiber(NDF)and acidic detergent fiber(ADF)contents of TX treatment were significantly lower than those of CK and LP treatment(P＜0.05).2)On opened bag 5 days,the pH and harmful microbial(yeast and mold)quantity of TX treat-ment were significantly higher than those of LP and CK treatments(P＜0.05).The carbon dioxide(CO2)gas production of CK treatment was significantly higher than that of LP and TX treatments(P＜0.05).The aerobic stability of CK and LP treatments was significantly higher than that of TX treatment(P＜0.05).3)On fermen-tation 10 and 35 days,the hemicellulase activity of TX treatment was significantly higher than that of CK treat-ment(P＜0.05).On fermentation 60 days,the amylase activity of each treatment was ranked as LP treatment＞TX treatment＞CK treatment(P＜0.05).4)On degradation 72 hours,the acid detergent fiber de-gradability(ADFD)of LP treatment was significantly higher than that of CK treatment(P＜0.05),and the or-ganic matter degradability(OMD)of CK treatment was significantly higher than that of LP and TX treatments(P＜0.05).5)The rapid degradation fraction,slow degradation fraction and potential degradation fraction of organic matter of CK and TX treatments were significantly higher than those of LP treatment(P＜0.05).In conclusion,the co-inoculation of CDB and homogeneous lactic acid bacteria improve the organic acid content and nutritional quality of silage maize,and improve the hemicellulase and amylase activities and organic matter degradability during fermentation process.[Chinese Journal of Animal Nutrition,2024,36(11):7185-7195]
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维普
