Effects of Replacing Soybean Meal with Fermented Rapeseed Brown Cake on Growth Performance,Meat Quality and Intestinal Health of Common Carp(Cyprinus carpio)
The purpose of this experiment was conducted to investigate the effects of adding different propor-tions of fermented rapeseed brown cake(FRBC)replacing part of soybean meal in diets on the growth per-formance,nutrient deposition rates,nutritional composition of whole fish and muscle,serum biochemical indi-ces and intestinal health of common carp.The principle of isonitrogen(42％crude protein)and isolipid(11％crude fat)was adopted.Diet containing 25％soybean meal was used as a control diet,and 8％,18％,27％and 36％soybean meal were replaced with FRBC,respectively.The proportions of FRBC in the corresponding diets were 0(FRBC0 group,as control group),3％(FRBC3 group),6％(FRBC6 group),9％(FRBC9 group),12％(FRBC12 group),respectively.A total of 2 450 juvenile common carp at average body weight of(99.97±1.33)g were randomly divided into 5 groups with 7 replicates per group and 70 fish per replicate,and fed with above 5 experimental diets for 8 weeks.The results showed as follows:1)the final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR)and protein efficiency ratio(PER)were sig-nificantly increased(P＜0.05),and the feed conversation ratio was significantly decreased(P＜0.05)when the proportion of FRBC was higher than or equal to 9％.There were no significant differences in survival rate,body indexes including condition factor,hepatosomatic index and viscerosomatic index among all groups(P＞0.05).2)Compared with the control group,adding different proportions of FRBC into diets had no significant effects on whole fish nutritional composition,such as crude protein,ether extract,crude ash and amino acids(P＞0.05),but significantly increased the content of calcium in muscle(P＜0.05).3)The deposition rates of protein,lysine and threonine were significantly increased(P＜0.05),and the lipid deposition rate was signifi-cantly decreased in the FRBC3,FRBC9 and FRBC12 groups compared with the control group(P＜0.05).4)Adding 12％FRBC into the diet significantly reduced the total cholesterol(TC)content and the activities of al-anine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum compared with the control group(P＜0.05).5)Compared with the control group,the total antioxidant capacity(T-AOC)in intestinal mucosa in the FRBC6 group was significantly increased(P＜0.05),but it was significantly decreased when the proportion of FRBC was higher than 9％(P＜0.05).6)The trypsin activity in intestinal mucosa in the FR-BC12 group was significantly higher than that in the control group(P＜0.05).7)No significant differences were observed in the mRNA relative expression levels of zonula occluden-1(ZO-1)and occlidin(Occlidin)a-mong groups(P＞0.05),but the mRNA relative expression level of claudin-1(Claudin-1)in the FRBC9 group was significantly down-regulated compared with the control group(P＜0.05).8)There were no signifi-cant differences in intestinal villus height and intestinal wall thickness among all groups(P＞0.05),and no ob-viously difference in intestinal tissue structure.Based on these results,in a diet containing 25％soybean meal,replacing 36％soybean meal with FRBC can promote growth performance,feed utilization rate and meat quali-ty of common carp without adverse effects on intestinal health.[Chinese Journal of Animal Nutrition,2024,36(11):7212-7226]
fermented rapeseed brown cakecommon carpgrowth performancenutrient utilizationintestinal health
万方数据
维普
