摘要
本试验旨在探究噬菌体制剂对育成期公貂生长性能、免疫功能及抗氧化能力的影响.选择96只75日龄育成期公貂,随机分为6组,每组8个重复,每个重复2只.对照组饲喂基础饲粮,试验组在基础饲粮中分别添加175、350、525、700和875 mg/kg的噬菌体制剂(大肠杆菌噬菌体和沙门氏菌噬菌体的复合制剂,二者比例为1:1,总效价为109PFU/g).试验期共8周,分为Ⅰ期(第1～4周)和Ⅱ期(第5～8周).结果表明:1)与对照组相比,饲粮中添加525、700和875 mg/kg的噬菌体制剂可显著或极显著降低试验Ⅰ期、试验Ⅱ期和试验全期料重比(F/G)和腹泻率(P＜0.05或P＜0.01),极显著提高试验Ⅱ期和试验全期平均日增重(ADG)(P＜0.01).2)与对照组相比,饲粮中添加700和875 mg/kg的噬菌体制剂可显著或极显著提高血清免疫球蛋白A(IgA)、免疫球蛋白G(IgG)和免疫球蛋白M(IgM)含量(P＜0.05或P＜0.01).3)与对照组相比,饲粮中添加700 mg/kg的噬菌体制剂可显著降低血清丙二醛(MDA)含量(P＜0.05),显著提高血清谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)(P＜0.05).4)与对照组相比,饲粮中添加875 mg/kg的噬菌体制剂可显著提高脾脏指数和肠体比(I/B)(P＜0.05),饲粮中添加525和700 mg/kg的噬菌体制剂可显著提高肾脏指数(P＜0.05).5)通过饲粮中噬菌体制剂添加水平与试验全期F/G、试验全期腹泻率、血清IgG含量、血清MDA含量拟合二次曲线,得出育成期公貂饲粮中噬菌体制剂的适宜添加水平为777.90-942.21 mg/kg.综合考虑,建议育成期公貂饲粮中噬菌体制剂添加水平为847.20 mg/kg.
Abstract
The aim of this study was to investigate the effects of bacteriophage preparation on growth perform-ance,immune function and antioxidant capacity of growing male minks.Ninety-six 75-day-old growing male minks were randomly divided into 6 groups with 8 replicates per group and 2 minks per replicate.Minks in the control group were fed a basal diet,and others in experimental groups were fed the basal diets supplemented with 175,350,525,700 and 875 mg/kg bacteriophage preparation(Escherichia coli bacteriophage and Sal-monella bacteriophage compound preparation,their ratio was 1∶1,and the total titer was 109 PFU/g),respec-tively.The experiment lasted for 8 weeks,and was divided into period Ⅰ(weeks 1 to 4)and period Ⅱ(weeks 5 to 8).The result showed as follows:1)compared with the control group,dietary 525,700 and 875 mg/kg bacteriophage preparation significantly decreased the feed to gain ratio(F/G)and diarrhea rate during experimental period Ⅰ,experimental period Ⅱ and whole experimental period(P＜0.05 or P＜0.01),significantly increased the average daily gain(ADG)during experimental period Ⅱ and whole experimental period(P＜0.01).2)Compared with the control group,dietary 700 and 875 mg/kg bacteriophage preparation significantly increased the contents of immunoglobulin A(IgA),immunoglobulin G(IgG)and immunoglobu-lin M(IgM)in serum(P＜0.05 or P＜0.01).3)Compared with the control group,dietary 700 mg/kg bacte-riophage preparation significantly decreased the serum malondialdehyde(MDA)content(P＜0.05),signifi-cantly increased the serum glutathione peroxidase(GSH-Px)activity and total antioxidant capacity(T-AOC)(P＜0.05).4)Compared with the control group,dietary 875 mg/kg bacteriophage preparation significantly in-creased the spleen index and intestine to body ratio(I/B)(P＜0.05),dietary 525 and 700 mg/kg bacterio-phage preparation significantly increased the kidney index(P＜0.05).5)The quadratic curves were fitted by dietary bacteriophage preparation supplemental level with F/G during whole experimental period,diarrhea rate during whole experimental period,serum IgG content,and obtained that the dietary optimum supplemental lev-el of bacteriophage preparation for growing male minks was 777.90 to 942.21 mg/kg.Comprehensive consid-eration,it is suggested that the dietary supplemental level of bacteriophage preparation for growing male minks is 847.20 mg/kg.[Chinese Journal of Animal Nutrition,2024,36(11):7284-7293]
维普
万方数据