The purpose of this experiment was to explore the effects of diarrhea on the composition of intestinal flora and drug resistance genes in forest musk deer.Three healthy forest musk deer(SZ)and three diarrhea forest musk deer(SF)aged 36 months were selected in the experiment.Feces of all individuals were collected and metagenomic sequencing analysis was performed.The results showed that the Shannon index of SF group decreased,and the abundances of beneficial bacteria Clostridium and Lactonifactor longoviformis were signifi-cantly lower than thoser of SZ group(P＜0.05),while the abundances of pathogenic bacteria Escherichia and Escherichia coli were significantly higher than those of SZ group(P＜0.05).The function of intestinal flora was further analyzed.In the analysis of CAZy function,the significant differential genes were from glycoside hydrolases,glycosyl transferases,polysaccharide lyases and carbohydrate-binding modules.In the CARD re-sistance gene analysis,the main resistance genes of forest musk deer were composed of 9 categories,among which the main resistance genes were rifamycins(45.65％),lincosamides(10.29％),and polypeptides(7.11％).There were three main phyla with more resistance genes,which were Firmicutes,Bacteroidetes and Proteobacteria.LEfSe analysis showed that the significant resistance genes in the SF group were more than those in the SZ group,mainly contributed by Proteobacteria.In summary,diarrhea can lead to a decrease in the diversity of intestinal flora,and an increase in intestinal pathogenic bacteria and resistance genes of forest musk deer,indicating that clinical treatment of bacterial diarrhea should pay attention to bacterial resistance.[Chinese Journal of Animal Nutrition,2024,36(11):7346-7357]
维普
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