摘要
本试验旨在优化油莎豆粕与中药复合物固态发酵工艺条件.采用贝莱斯芽孢杆菌对油莎豆粕和中药复合物进行固态发酵,以多糖和蛋白质提取率为指标,通过单因素试验对发酵温度、料液比、接种量、发酵时间、碳酸钙(CaCO3)添加量、油莎豆粕与中药添加比进行初步优化,再通过Plackett-Burman试验筛选出3个显著(P＜0.05)影响油莎豆粕与中药复合物中多糖和蛋白质提取率的因素,结合最陡爬坡试验确定最大响应值,运用Box-Behnken试验确定最优工艺条件.结果表明:最优工艺条件为发酵温度40 ℃、料液比1.0∶2.0 g/mL、接种量2％、发酵时间41 h、CaCO3添加量0.002 g/mL、油莎豆粕与中药添加比1.0∶2.5 g/g;与未发酵前相比,油莎豆粕与中药复合物中多糖和蛋白质提取率分别提高了 2.65倍与2.14倍.由此可见,优化后的固态发酵工艺条件可有效提高油莎豆粕与中药复合物中多糖和蛋白质提取率,为油莎豆粕与中药复合物在畜牧业中的开发应用提供理论依据.
Abstract
The purpose of this experiment was to optimize the solid-state fermentation conditions of the Cyperus esculentus soybean meal and traditional Chinese medicine compound.The Bacillus velezensis was used for sol-id-state fermentation of Cyperus esculentus soybean meal and traditional Chinese medicine compound,with ex-traction rates of polysaccharide and protein as indicators,the fermentation temperature,material-liquid ratio,inoculation amount,fermentation time,calcium carbonate(CaCO3)addition and addition ratio of Cyperus es-culenta soybean meal to traditional Chinese medicine were preliminarily optimized by single factor experiment.Then,three significant(P＜0.05)factors affecting extraction rates of polysaccharide and protein in Cyperus es-culentus soybean meal and traditional Chinese medicine compound were screened out by Plackett-B urman ex-periment,the maximum was determined by steepest climbing test,and used Box-Behnken experiment to deter-mine the optimal technological conditions.The results showed that the optimal technological conditions were fermentation temperature 40 ℃,material-liquid ratio 1.0∶2.0 g/mL,inoculation amount 2％,fermentation time 41 h,CaCO3 addition 0.002 g/mL and the addition ratio of Cyperus esculenta soybean meal to traditional Chinese medicine 1.0∶2.5 g/g.Compared with before fermentation,the extraction rates of polysaccharide and protein in the Cyperus esculentus soybean meal and traditional Chinese medicine compound were increased by 2.65 times and 2.14 times,respectively.In conclusion,the optimized solid-state fermentation conditions can effectively improve extraction rates of polysaccharide and protein in the Cyperus esculentus soybean meal and traditional Chinese medicine compound,to provide theoretical basis for the development and application of the Cyperus esculentus soybean meal and traditional Chinese medicine compound in animal husbandry.[Chinese Journal of Animal Nutrition,2024,36(11):7358-7370]
维普
万方数据