Optimization of Solid-State Fermentation Process Conditions of Cyperus esculentus Soybean Meal and Traditional Chinese Medicine Compound
The purpose of this experiment was to optimize the solid-state fermentation conditions of the Cyperus esculentus soybean meal and traditional Chinese medicine compound.The Bacillus velezensis was used for sol-id-state fermentation of Cyperus esculentus soybean meal and traditional Chinese medicine compound,with ex-traction rates of polysaccharide and protein as indicators,the fermentation temperature,material-liquid ratio,inoculation amount,fermentation time,calcium carbonate(CaCO3)addition and addition ratio of Cyperus es-culenta soybean meal to traditional Chinese medicine were preliminarily optimized by single factor experiment.Then,three significant(P＜0.05)factors affecting extraction rates of polysaccharide and protein in Cyperus es-culentus soybean meal and traditional Chinese medicine compound were screened out by Plackett-B urman ex-periment,the maximum was determined by steepest climbing test,and used Box-Behnken experiment to deter-mine the optimal technological conditions.The results showed that the optimal technological conditions were fermentation temperature 40 ℃,material-liquid ratio 1.0∶2.0 g/mL,inoculation amount 2％,fermentation time 41 h,CaCO3 addition 0.002 g/mL and the addition ratio of Cyperus esculenta soybean meal to traditional Chinese medicine 1.0∶2.5 g/g.Compared with before fermentation,the extraction rates of polysaccharide and protein in the Cyperus esculentus soybean meal and traditional Chinese medicine compound were increased by 2.65 times and 2.14 times,respectively.In conclusion,the optimized solid-state fermentation conditions can effectively improve extraction rates of polysaccharide and protein in the Cyperus esculentus soybean meal and traditional Chinese medicine compound,to provide theoretical basis for the development and application of the Cyperus esculentus soybean meal and traditional Chinese medicine compound in animal husbandry.[Chinese Journal of Animal Nutrition,2024,36(11):7358-7370]
solid-state fermentationprocess optimizationCyperus esculentus soybean mealtraditional Chi-nese medicine
万方数据
维普
