Effects of Wilting and Additive Treatment on Quality and Protein Fractions of Avena sativa Silage
This study aimed to explore the effects of wilting and additives(citric acid,L-malic acid and Lacto-bacillus plantarum)on the quality and protein fractions of Avena sativa silage.Distilled water(Con),Lacto-bacillus plantarum(LP),citric acid(CA),L-malic acid(MA),Lactobacillus plantarum and citric acid mixture(LP+CA),and Lactobacillus plantarum and malic acid mixture(LP+MA)were added to unwilted and wilted Avena sativa raw materials and fermented for 45 days.The results showed that all the additives sig-nificantly decreased the pH of Avena sativa silage(P<0.05).The addition of CA and MA alone significantly decreased the lactic acid contents(P<0.05).The compound additive composed of CA,MA and LP signifi-cantly decreased the pH,ammonia nitrogen and amine nitrogen contents of Avena sativa silage(P<0.05).A-mong all treatments,water soluble carbohydrate content in the CA and MA was higher than other treatments.The additives had no significant effects on neutral detergent fiber,acid detergent fiber,hemicellulose and crude protein contents of Avena sativa silage(P>0.05).The biogenic amines in Avena sativa silage were mainly pu-trescine,cadaverine and tyramine,accounting for about 90%of the total biogenic amines measured.The con-tents of phenethylamine,putrescine,cadaverine,histamine,tyramine and total biogenic amines in the additive treatment group were significantly lower than that in the Con(P<0.05).The contents of putrescine,cadaver-ine,tyramine and total biogenic amines decreased significantly in the LP+CA and LP+MA.Compared with un-wilted oat silage,the free amino acid nitrogen content of wilted oat silage was significantly decreased(P<0.05),but the ammonia nitrogen content was significantly increased(P<0.05).Therefore,LP+CA and LP+MA can improve the fermentation quality of Avena sativa silage,effectively inhibit protein degradation,and play an important role in improving the quality and safety of Avena sativa silage.[Chinese Journal of Animal Nutrition,2024,36(11):7371-7382]