Research on Biological Characteristics of Co-Fermentation Products of Swine-Derived Lactobacillus plantarum and Eucommia ulmoides Leaf Polysaccharides
The aim of this experiment was to study the biological characteristics of the co-fermentation products of a strain of swine-derived Lactobacillus plantarum(S.L.plantarum)and Eucommia ulmoides leaf polysac-charides(ELP),and to explore the possibility of using it as a feed additive.Firstly,three strains of probiotics were isolated and identified from fresh feces of healthy pigs,and the probiotic with the best antibacterial activi-ty and growth activity was selected for co-fermentation with ELP.Then,the optimal fermentation time was de-termined based on the strength of antibacterial effects.At the same time,the effects of temperature,pH and pepsin treatment on the antibacterial activity and the effects of artificial gastrointestinal fluid treatment on the vi-able bacteria count of the co-fermentation products were measured.Finally,the acute oral toxicity experiment of the co-fermentation liquid was carried out.The results showed as follows:1)among the three probiotics,S.L.plantarum had the highest viable bacteria number and the strongest antibacterial activity,and co-fermentation with ELP significantly increased its viable bacteria number(P＜0.05),while significantly improved its antibac-terial activity(P＜0.05).Based on this,the optimal co-fermentation time of S.L.plantarum and ELP was con-firmed to be 12 to 24 h.2)Temperature had no significant effect on the antibacterial activity of the co-fermen-tation products(P＞0.05),but high pH(pH＞4)and high concentration of pepsin inhibited the antibacterial activity of the products,suggesting that the possible material basis of the antibacterial activity was organic acids and small molecule polypeptides.3)The gastrointestinal tolerance of S.L.plant arum was significantly im-proved by co-fermentation of S.L.plantarum and ELP,and the co-fermentation solution had no acute toxicity to mice.In conclusion,the co-fermentation of S.L.plantarum and ELP can significantly improve its antibacte-rial activity and gastrointestinal tolerance,which is suitable for oral administration,and has the possibility of developing into a new antibacterial feed additive.[Chinese Journal of Animal Nutrition,2024,36(11):7422-7434]
swine-derived Lactobacillus plantarumisolation and identificationEucommia ulmoides leaf pol-ysaccharidesco-fermentationantibacterial activity
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