Effects of Callicarpa nudiflora Extract on Growth Performance,Immune Function,Anti-Inflammatory Ability and Intestinal Barrier Function of Chickens Infected with Escherichia coli
This experiment was conducted to study the effects of Callicarpa nudiflora extract on growth per-formance,immune function,anti-inflammatory ability and intestinal barrier function of chickens infected with Escherichia coli.A total of 300 healthy 1-day-old white feather chickens were randomly divided into 4 groups with 3 replicates per group and 25 chickens per replicate.Chickens in control group(CON group)and Esche-richia coli infection group(EC group)were fed basal diets,and others in low and high dose Callicarpa nudi-flora extract groups were fed basal diets supplemented with 0.3(CNEL group)and 0.5 g/kg(CNEH group)Callicarpa nudiflora extract,respectively.On 19 days of age,chickens in EC,CNEL and CNEH groups were intraperitoneally injected with 1.0 mL Escherichia coli with a concentration of 1×10 8 CFU/mL,and chickens in the CON group were intraperitoneally injected with 1.0 mL normal saline with a concentration of 0.9％.The experiment lasted for 21 days.The results showed as follows:1)after infected with Escherichia coli,com-pared with the CON group,the average daily gain(ADG)of EC group was significantly decreased(P＜0.05),and the feed conversion rate(FCR)and mortality rate(DR)were significantly increased(P＜0.05).Compared with the EC group,the ADG of CNEL and CNEH groups was significantly increased(P＜0.05),and the FCR and DR were significantly decreased(P＜0.05).2)Compared with the CON group,the liver in-dex and spleen index of EC group were significantly increased(P＜0.05),and the bursa of Fabricius index was significantly increased(P＜0.05).Compared with the EC group,the liver index and spleen index of CNEL and CNEH groups were significantly decreased(P＜0.05),and the bursa of Fabricius index of CNEH group was significantly increased(P＜0.05).3)Compared with the CON group,the serum interleukin-6(IL-6)content of EC group was significantly increased(P＜0.05),and serum interleukin-10(IL-10)content was significantly decreased(P＜0.05).Compared with the EC group,the serum IL-6 and tumor necrosis fac-tor-α(TNF-α)contents of CNEL and CNEH groups were significantly decreased(P＜0.05),and the serum IL-10 content of CNEH group was significantly increased(P＜0.05).4)Compared with the CON group,the mRNA relative expression levels of IL-10 in jejunum and interleukin-4(IL-4),IL-10,B-cell lymphoma-2(Bcl-2)in spleen of EC group were significantly decreased(P＜0.05),and the mRNA relative expression lev-els of TNF-α,myeloid differentiation protein 88(MyD88),B-cell lymphoma 2-associated X protein(Bax),cysteinyl aspartate specific proteinase 3(Caspase 3),cysteinyl aspartate specific proteinase 8(Caspase 8)in jejunum and IL-6,TNF-α,interferon-γ(IFN-γ),Toll-like receptor 4(TLR4),Bax,Caspase 8 in spleen were significantly increased(P＜0.05).Compared with the EC group,the mRNA relative expression levels of IL-1β,TNF-α,Caspase 3,Caspase 8 in jejunum and MyD88 in spleen of CNEL and CNEH groups were sig-nificantly decreased(P＜0.05),and the mRNA relative expression levels of IL-4,IL-10 in jejunum and IL-4,IL-10 in spleen were significantly increased(P＜0.05).5)Compared with the CON group,the serum D-lactic acid(D-LA)and lipopolysaccharide(LPS)contents of EC group were significantly increased(P＜0.05).Compared with the EC group,the serum D-LA and LPS contents of CNEH group were significantly decreased(P＜0.05),and the serum diamine oxidase(DAO)content of CNEL and CNEH groups were significantly de-creased(P＜0.05).6)Compared with the CON group,the mRNA relative expression levels of zonula occlu-dens protein-1(ZO-1)and Claudin in jejunum of EC group were significantly decreased(P＜0.05).Compared with the EC group,the mRNA relative expression levels of ZO-1 and Occludin in jejunum of CNEL and CNEH groups were significantly increased(P＜0.05),and the mRNA relative expression level of Claudin in jejunum of CNEH group was significantly increased(P＜0.05).7)Compared with the CON group,the liver Esche-richia coli number of EC group was significantly increased(P＜0.05).Compared with the EC group,the liver Escherichia coli number of CNEL and CNEH groups was significantly decreased(P＜0.05).In conclusion,di-etary Callicarpa nudiflora extract can improve the effects of Escherichia coli infection on growth performance of chickens,and reduce the DR.In addition,it can improve the organ indexes of chickens,enhance the body anti-apoptosis ability,regulate the serum immune indexes and partially regulate the expression of TLR4/MyD88/nuclear factor-κB(NF-κB)pathway and related inflammatory factors to resist the inflammatory re-sponse caused by Escherichia coli infection,and improve the intestinal morphology and bacterial translocation.The Callicarpa nudiflora extract can be used as a potential substitute for antimicrobials in poultry production,and the optimal dose is 0.5 g/kg.[Chinese Journal of Animal Nutrition,2024,36(12):7754-7769]
Callicarpa nudiflora extractchickensEscherichia coliimmuneanti-inflammatoryintestinal barrier function
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