Study on Metabolic Differences of Xuanhan Yellow Cattle,Simmental Crossbred Cattle and Cattle-Yak Based on Metabolomics
This study was to determine the metabolic differences of Xuanhan yellow cattle,Simmental cross-bred cattle and cattle-yak.Eight castrated bulls with the same age(22 months of age),similar body condition and no disease,Xuanhan yellow cattle,Simmental crossbred cattle(Simmental×Xuanhan yellow cattle)and cattle-yak(Jersey×Maiwa yak)were selected as experimental subjects under the same diet conditions for 120 days.The serum and 24-hour urine of three types of beef cattle were collected,and the metabolites in serum and urine were determined by gas chromatography-mass spectrometry(GC-MS)method.The differential me-tabolites in serum and urine were obtained by principal component analysis(PCA)and partial least squares dis-criminant analysis(PLS-DA),and finally metabolic pathway analysis was performed by KEGG database.The results showed as follows:1)the dry matter intake(DMI)and average daily gain(ADG)of Simmental crossbred cattle were significantly higher than those of Xuanhan yellow cattle and cattle-yak(P＜0.05);the feed intake per unit of body weight of cattle-yak was significantly higher than that of Xuanhan yellow cattle and Simmental crossbred cattle(P＜0.05),and the feed/gain ratio of cattle-yak was significantly higher than that of Simmental crossbred cattle(P＜0.05).The glutathione peroxidase(GSH-Px)activity and total antioxidant capacity(T-AOC)in serum of Xuanhan yellow cattle were significantly higher than those of Simmental cross-bred cattle and cattle-yak(P＜0.05).2)The concentrations of threonine,methionine,urea,etc.in serum of cattle-yak were significantly higher than those odf Simmental crossbred cattle[variable importance in the pro-jection(VIP)＞1.0 and P＜0.05],the concentrations of glutamine,2-amino-butyric acid,urea,etc.in serum of cattle-yak were significantly higher than those of Xuanhan yellow cattle(VIP＞1.0 and P＜0.05),and the concentrations of eicosanoic acid,arachidonic acid,nonadecanoic acid,etc.in serum of cattle-yak were signif-icantly higher than those of Simmental crossbred cattle(VIP＞1.0 and P＜0.05);in addition,the concentra-tions of oleic acid,linoleic acid,octadecanoic acid,etc.in urine of cattle-yak were significantly higher than those of Xuanhan yellow cattle(VIP＞1.0 and P＜0.05).The concentrations of α-tocopherol,γ-tocophero,fu-maric acid in serum and succinic acid in urine of Xuanhan yellow cattle were significantly higher than those of Simmental crossbred cattle and cattle-yak(VIP＞1.0 and P＜0.05),and the concentration of uric acid in urine of Xuanhan yellow cattle was significantly higher than that of Simmental crossbred cattle and cattle-yak(VIP＞1.0 and P＜0.05);the concentrations of citric acid and α-ketoglutaric acid of cattle-yak were significantly high-er than those of Simmental crossbred cattle(VIP＞1.0 and P＜0.05).In conclusion,amino acid and lipid catab-olisms are higher in the cattle-yak comparison with the Xuanhan yellow cattle and Simmental crossbred cattle,the antioxidant capacity and purine and vitamin E metabolism are higher in the Xuanhan yellow cattle compari-son with the Simmental crossbred cattle and cattle-yak,and the order of the tricarboxylic acid cycle is:Xuan-han yellow cattle＞cattle-yak＞Simmental crossbred cattle.[Chinese Journal of Animal Nutrition,2024,36(12):7840-7853]
万方数据
维普
