The aim of this experiment was to investigate the effects of dietary Lactobacillus reuteri LRA7 on health condition,nutrient apparent digestibility,immune function and antioxidant capacity of beagle dogs.Thir-ty-two adult healthy Beagles with similar body weight were selected and randomly divided into four groups with eight dogs in each group.Dogs in the control group(CON group)were fed a basal diet,and others in probiot-ic addition groups were fed basal diets supplemented with 2×10 8,2×10 9 and 2×10 10 CFU/d Lactobacillus reu-teri LRA7,respectively.The pre-test period was 7 d,and the formal period was 28 d.The results showed as follows:1)there were no significant differences in health indices such as body temperature,respiratory rate,pulse,fecal scores,growth performance and blood routine of dogs(P>0.05).2)There was no significant difference in dry matter,crude protein,crude ash,and carbohydrate apparent digestibility of dogs(P>0.05).The ether extract apparent digestibility of MR group was significantly higher than that of the CON and LR groups(P<0.05).3)The serum urea nitrogen(UN)content of HR group was significantly lower than that of other groups(P>0.05),and the serum secretory immunoglobulin A(sIgA)content was significantly higher than that of CON and LR groups(P<0.05).4)The serum superoxide dismutase(SOD)activity of MR and HR groups was significantly higher than that of CON and LR groups(P<0.05),the serum glutathione peroxi-dase(GSH-Px)activity of LR,MR and HR groups was significantly higher than that of CON group(P<0.05),and the serum malondialdehyde(MDA)content of HR group was significantly lower than that of other groups(P<0.05).In conclusion,Lactobacillus reuteri LRA7 has no adverse effects on the growth and health status of beagle dogs,can increase the ether extract apparent digestibility,improve immune function and an-tioxidant capacity,and this strain can be used as a candidate strain for probiotic products of canine origin.[Chi-nese Journal of Animal Nutrition,2024,36(12):7995-8004]