Effects of Bacillus subtilis on Intestinal Nutrient Transporter Gene Expression of Lipopolysaccharide in Broilers
The aim of this experiment was to study the gene expression of intestinal nutrient transporter of broil-ers under lipopolysaccharide(LPS)stress using Bacillus subtilis as feed additive.A 2×2 two-factor design was adopted,factor 1 was whether Bacillus subtilis was added or not(0 and 200 g/t),and factor 2 was whether LPS was stressed or not(0 and 500 μg/kg BW).A total of 192 healthy 1-day-old Arbor Acres broilers(male)with similar body weight were selected.They were randomly divided into 4 groups with 6 replicates per group and 8 chickens in per replicate.Broilers in control group were fed a basal diet and given normal saline through oral administration.Broilers in experimental group Ⅰ were fed the basal diet and given LPS through o-ral administration.Broilers in experimental group Ⅱ were fed the basal diet supplemented with Bacillus subtilis and given normal saline through oral administration.Broilers in experimental group Ⅲ were fed the basal diet supplemented with Bacillus subtilis and given LPS through oral administration.The experiment was divided into three stages of pre-stress(days 1 to 14),LPS stress(days 15 to 21)and stress recovery(days 22 to 28),with a total of 28 days.Duodenum,jejunum and ileum samples were collected.Subsequently,the relevant mRNA was extracted from the samples and reverse-transcribed,and the expression of genes detected by fluo-rescent quantitative PCR.The results showed as follows:on day 21 of the experiment,LPS stress significantly reduced the mRNA relative expression levels of B0 system neutral amino acid transporter 1(B0AT1),sodium-dependent glucose transporter 1(SGLT1)and fatty acid transporter 4(FATP4)in the whole intestine of broil-ers(P＜0.05);there was a significant interaction between dietary supplementation of Bacillus subtilis and LPS stress on B0AT1 mRNA relative expression level in duodenum of broilers(P＜0.05);dietary supplementation of Bacillus subtilis significantly increased the mRNA relative expression levels of SGLT1 and FATP4 in jejunum of broilers(P＜0.05).On day 28 of the experiment,dietary supplementation of Bacillus subtilis significantly increased the mRNA relative expression level of B0AT1 in ileum of broilers(P＜0.05).In conclusion,LPS stress can down-regulate the expression of intestinal nutrient transporter genes in broilers,and dietary supple-mentation of BS can up-regulate the expression of intestinal nutrient transporter genes in broilers,those results indicate that Bacillus subtilis can alleviate the negative effect of LPS stress on intestinal nutrient transport in broilers,and enable normal expression of intestinal nutrient transporter genes in broilers.[Chinese Journal of Animal Nutrition,2024,36(12):8014-8024]
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维普
