Silent Information Regulator 2 Related Enzyme 3 Ameliorated Oxidative Stress and Inflammatory Response of Dairy Cows by Enhance Mitochondrial Function
The aim of this experiment was to investigate the role of silent information regulator 2 related en-zyme 3(SIRT3)in oxidative stress-induced mastitis in dairy cows,and to provide theoretical basis and techni-cal support for the prevention and control of mastitis in dairy cows.The experiment was divided into cows in vi-vo experiment and cell in vitro experiment,the blood and milk samples were collected from 10 healthy cows and 10 mastitis cows in late lactation[(200±20)d]with similar body condition scores(3.0 to 3.5 scores)and parities(2 to 3 parities),to investigate the changes in mitochondrial function,oxidative stress and inflam-matory response.After the overexpression and interference of SIRT3,the hydrogen peroxide(H2O2)was used to damage bovine mammary epithelial cells(BMECs)to determine the effects of SIRT3 on the inflammatory response of oxidation-damaged BMECs.The results showed as follows:1)the contents of glutathione peroxi-dase(GSH-Px),nuclear respiratory factor 1(NRF1),peroxisome proliferator-activated receptor γ coactiva-tor-1α(PGC-1α),SIRT3 and mitochondrial transcription factor A(TFAM)in plasma of mastitis cows were significantly lower than those of healthy cows(P＜0.01),and the contents of malondialdehyde(MDA),inter-leukin-6(IL-6)and interleukin 8(IL-8)in plasma of mastitis cows were significantly higher than those of healthy cows(P＜0.05 or P＜0.01).2)The interfering SIRT3(si-SIRT3)followed by H2O2 treatment signifi-cantly up-regulated the mRNA relative expression levels of tumor necrosis factor-α(TNF-α),interleukin 1β(IL-1β),IL-6,IL-8,cysteinyl aspartate specific proteinase-3(CASPASE-3)and cysteinyl aspartate specific proteinase-9(CASPASE-9)and fluorescence intensity of reactive oxygen species(ROS)in BMECs(P＜0.05 or P＜0.01),and significantly down-regulated the mRNA relative expression levels of superoxide dismutase(SOD),GSH-Px,PGC-1α and TFAM in BMECs(P＜0.05 or P＜0.01),induced oxidative stress,mitochon-drial damage,inflammatory response and apoptosis in BMECs.While overexpression of SIRT3(oe-SIRT3)significantly reversed the above responses(P＜0.05 or P＜0.01).In conclusion,the expression of SIRT3 is sig-nificantly down-regulated in mastitis cows,and overexpression of SIRT3 in vitro can alleviate oxidative stress and inflammatory responses of BMECs and inhibit apoptosis of BMECs by regulating mitochondrial function,which provides technical support for the enhancement of mammary health of dairy cows through the activation of SIRT3 in production.[Chinese Journal of Animal Nutrition,2024,36(12):8049-8061]
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维普
