Effects of Resveratrol on Mitochondrial Damage,Oxidative Stress,Inflammatory Damage and Cell Apoptosis of Hepatocytes Induced by Non-Esterified Fatty Acids of Dairy Cows
The aim of this study was to investigate the protective effects of resveratrol(RES)on hepatocyte damage induced by non-esterified fatty acids(NEFA)of dairy cows.There were four experimental groups,namely the control group(CON group,incubated hepatocytes with starvation medium for 12 hours),NEFA group(incubated hepatocytes with starvation medium contained 1.2 mmol/L NEFA for 12 hours),RES group(incubated hepatocytes with starvation medium contained 20 µmol/L RES for 12 hours)and RES+NEFA group(incubated hepatocytes with starvation medium contained 1.2 mmol/L NEFA+20 µmol/L RES for 12 hours),respectively.The results showed as follows:the mRNA relative expression levels of peroxisome proliferator-activated receptor y coactivator-1α(PGC-1α),mitochondrial transcription factor A(TFAM),su-peroxide dismutase(SOD),glutathione peroxidase(GSH-Px)and B-cell lymphoma-2(Bcl-2)in hepatocytes of NEFA groups were significantly lower than those of CON group(P＜0.05 or P＜0.01),and the mRNA rela-tive expression levels of nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),interleu-kin-6(IL-6),interleukin-8(IL-8),cysteinyl aspartate specific proteinase-3(Caspase-3)and cysteinyl aspar-tate specific proteinase-9(Caspase-9),reactive oxygen species(ROS)and cell apoptosis rate in hepatocytes were significantly higher than those of CON group(P＜0.01);and adding RES could significantly reversed the above responses(P＜0.05 or P＜0.01).In conclusion,the addition of RES can alleviate mitochondrial function damage,cellular oxidative stress,inflammatory response and cell apoptosis of hepatocytes of daily cows.[Chi-nese Journal of Animal Nutrition,2024,36(12):8062-8071]
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