Screening,Identification and Characterization of Deoxynivalenol Degrading Bacteria
The aim of this study was to screen and isolate a strain from moldy maize capable of degrading de-oxynivalenol(DON)and to study its characteristics.Using DON as the sole carbon source,the target strains were screened and isolated from moldy maize by enrichment culture and line separation techniques,and the degradation rate of DON by different strains was detected by enzyme-linked immunosorbent assay(ELISA)in 24 h.A strain with good degradation effect was screened and named BT-11.Morphological observation,physi-ological and biochemical tests and 16S rRNA and gyrB sequence comparison were conducted to identify the strain BT-11.The optimal growth and degradation conditions of BT-11 were investigated by optimizing incuba-tion time,temperature,pH,inoculum volume and toxin concentration,and the degradation mechanism of BT-11 was initially investigated.The results showed as follows:1)a strain with good degradation effect was suc-cessfully isolated from moldy maize,and the degradation rate of DON was(80.07±0.96)％within 24 h.2)The strain BT-11 was identified as Bacillus subtilis,and its optimal growth and degradation time,temperature and pH were 24 h,37 ℃ and 7.0 respectively,meanwhile it also had good bioactivity under the condition of 5％inoculum,and it had a certain toxin tolerance under the environment of DON concentration ranging from 2 to 10 mg/L.The main active substance for DON degradation by BT-11 was hypothesized to be a heat-resistant extracellular enzyme by the active substance characterization test.In conclusion,a strain Bacillus subtilis that can efficiently degrade DON is isolated from moldy corn in this study,which can broaden the candidate strains for degrading DON microorganisms,and provide a theoretical basis for the use of Bacillus subtilis to treat DON contaminated feed in livestock and poultry production.[Chinese Journal of Animal Nutrition,2024,36(12):8137-8148]
deoxynivalenolbiodegradationBacillusmycotoxinsisolation and identification
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维普
