Macrogenomic Analysis Reveals Gut Microbial Community Characteristics of Healthy Captive Sichuan,Yunnan and Guizhou Golden Snub Nosed Monkeys
Based on macrogenomic technology,the gut microbial community composition and their functions in captive Sichuan(Rhinopithecus roxellana),Yunnan(Rhinopithecus bieti)and Guizhou golden snub nosed monkeys(Rhinopithecus brelichi)were compared in this experiment.Fecal samples of three Rhinopithecus roxellana,three Rhinopithecus bieti and four Rhinopithecus brelichi were collected before morning feeding for metagenomic sequencing.The results showed that the gut microbial community of Rhinopithecus roxellana had lower abundance,homogeneity and diversity than those of Rhinopithecus bieti and Rhinopithecus brelichi at the genus level in the α diversity analysis.In the gut microbial community of the three species of captive golden snub-nosed monkeys,the dominant phyla were Firmicutes,Bacteroidota and Uroviricota,and the dominant genera were Alistipes,unclassified_f_Oscillospiraceae,unclassified_c_Clostridia;the results of principal com-ponent analysis(PCA)showed that the gut microbial community composition of the three species of captive golden snub-nosed monkeys varied greatly at the genus level.The results of differential test in gut microbial community at the phylum level and genus level and the analysis of functional differences indicated that higher metabolic activity in the gut microbiome of both Rhinopithecus brelichi and Rhinopithecus roxellana,whereas all three species of captive golden snub-nosed monkeys had the similar ability to metabolize carbohydrates.A total of 10 microbial markers with significant differences in abundance[linear discriminant analysis(LDA)score＞3]were detected in this experiment,among which 5 belonged to Candidatus_Aenigmarchaeota and 5 belonged to Candidatus_Lokiarchaeota in Rhinopithecus bieti and 5 belonged to Rhinopithecus brelichi.In terms of functional prediction,the genes related to metabolic functions were the most abundant in the three species of captive golden snub-nosed monkeys,with the highest abundance of global and overview maps and the second of carbohydrate metabolism,which was in line with the leaf-feeding characteristics of golden snub nosed mon-keys.This study provides information on the feeding and nutritional metabolism of captive golden snub nosed monkeys.[Chinese Journal of Animal Nutrition,2024,36(12):8149-8160]
万方数据
维普
