Rapid Detection of SARS-CoV-2 Using an Innovative"Magic Strip"
Colloidal gold,a marker widely embraced in commercial point-of-care testing(POCT)for immunochromatographic assays,is distinguished by its vivid color characteristics.It is environmentally benign,morphologically stable,and required no further visual modifications.This study details a straightforward process for fabricating test strips that obviate the need for sophisticated,costly equipment.Colloidal gold,synthesized by reducing the chloroauric acid with trisodium citrate,was conjugated to the nucleocapsid N protein antibody of SARS-CoV-2.With the test strip's visible area,a competitive antibody-antigen-antibody sandwich complex forms,with the red test line serving as the basis for qualitative analysis of the virus.This detection approach is visually discernible,user-friendly,rapid(within 10 min),sensitive(detection limit of 0.5 ng∙mL-1)and cost-effective(each strip costing no more than 5 yuan).The experimental process offers students an opportunity to engage with the specific biochemical reactions triggered by antigen-antibody interactions,fostering a deep interest in exploring rapid diagnostic technologies that integrate chemistry,biology,life sciences and nanomaterials.
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