Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism
Two protocols,the transient method and the mathematical method,are introduced to deduce all the rate constants in the enzyme-catalyzed reaction model based on the Michaelis-Menten mechanism.The transient method focuses on analyzing the period before reaching a steady state,while the mathematical method aims to establish a relationship between the concentration of the enzyme and the substrate.By calculating all the rate constants,one can enhance their understanding and grasp of the kinetics of the enzyme-catalyzed reaction.
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