基于米氏机理的酶催化反应中反应速率常数的计算
Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism
张恒1
作者信息
- 1. 武汉大学化学与分子科学学院,武汉 430072
- 折叠
摘要
对基于米氏机理的酶催化反应模型,本文介绍了求解全部反应速率常数的两种方法:瞬态法和数学计算法,其中瞬态法的核心是分析反应达到稳态前的过程,数学计算法的核心是寻找反应过程中酶与底物浓度之间的关系.通过求解全部反应速率常数,不仅可以加深对酶催化反应动力学的理解,而且有助于提高对反应动力学的认识.
Abstract
Two protocols,the transient method and the mathematical method,are introduced to deduce all the rate constants in the enzyme-catalyzed reaction model based on the Michaelis-Menten mechanism.The transient method focuses on analyzing the period before reaching a steady state,while the mathematical method aims to establish a relationship between the concentration of the enzyme and the substrate.By calculating all the rate constants,one can enhance their understanding and grasp of the kinetics of the enzyme-catalyzed reaction.
关键词
酶催化反应/动力学/米氏机理/速率常数Key words
Enzyme catalyzed reaction/Kinetics/Michaelis-Menten mechanism/Rate constant引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据