Experimental Teaching Design on Synthesis and Antitumor Activity Study of Cu-Pyropheophorbide-a Methyl Ester
In this experiment,Cu-pyropheophorbide-a methyl ester (Cu-MPPa) was designed to be synthesized for cancer therapy mediated by reactive oxygen species (ROS) and glutathione depletion. Additionally,it facilitates the maintenance of high intracellular oxygen concentrations through cyclic oxygen production via a Fenton-like reaction. The reactive oxygen generation capacity,oxygen production capacity and glutathione consumption capacity of Cu-MPPa were investigated during the experiment. Mass spectrometry was used to characterize the structures of the synthesized intermediates. Combining Organic Chemistry synthesis,Instrumental Analytical Chemistry and Biochemistry experiments,this experiment necessitates a team of three students and is estimated to take about 24 credit hours. It is designed to cultivate students' abilities in synthesis and innovation,complex problem-solving and teamwork enhancement.
Cu-pyropheophorbide-a methyl esterReactive oxygen species (ROS)Organic chemical synthesisInstrumental analysisAntineoplastic
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