铜-焦脱镁叶绿酸a甲酯的合成及抗肿瘤活性研究实验教学设计
Experimental Teaching Design on Synthesis and Antitumor Activity Study of Cu-Pyropheophorbide-a Methyl Ester
宋平 1张楠 1王洁 1闫蕊 1王志强 1金英学1
作者信息
- 1. 哈尔滨师范大学化学化工学院,哈尔滨 150025
- 折叠
摘要
本实验设计合成了铜-焦脱镁叶绿酸a甲酯(Cu-MPPa),用于活性氧(ROS)介导的癌症治疗和消耗谷胱甘肽,并通过类Fenton反应循环产氧维持细胞内高浓度氧.实验过程中考察了Cu-MPPa的活性氧产生能力、氧气产生能力和谷胱甘肽消耗能力等性能.所合成中间体的结构用质谱进行了表征.这个实验综合了有机化学合成、仪器分析化学和生物化学实验,要求由三名学生组成实验小组,大约需要24学时,旨在培养学生综合创新能力、解决复杂问题能力和培养团队合作精神.
Abstract
In this experiment,Cu-pyropheophorbide-a methyl ester (Cu-MPPa) was designed to be synthesized for cancer therapy mediated by reactive oxygen species (ROS) and glutathione depletion. Additionally,it facilitates the maintenance of high intracellular oxygen concentrations through cyclic oxygen production via a Fenton-like reaction. The reactive oxygen generation capacity,oxygen production capacity and glutathione consumption capacity of Cu-MPPa were investigated during the experiment. Mass spectrometry was used to characterize the structures of the synthesized intermediates. Combining Organic Chemistry synthesis,Instrumental Analytical Chemistry and Biochemistry experiments,this experiment necessitates a team of three students and is estimated to take about 24 credit hours. It is designed to cultivate students' abilities in synthesis and innovation,complex problem-solving and teamwork enhancement.
关键词
铜-焦脱镁叶绿酸a甲酯/活性氧/有机化学合成/仪器分析/抗肿瘤Key words
Cu-pyropheophorbide-a methyl ester/Reactive oxygen species (ROS)/Organic chemical synthesis/Instrumental analysis/Antineoplastic引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据