Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography
This experiment aims to design novel stationary phases for efficient separation of various analytes and apply them in teaching experiments.Covalent organic frameworks(COFs)were coated onto silica microspheres to synthesize core-shell structured composites(SiO2@COF),which were then employed as a mixed-mode stationary phase for high performance liquid chromatography(HPLC).Thanks to the excellent properties of the covalent organic framework,the alkylbenzenes,anilines,phenols,sulfonamides,nucleosides and polycyclic aromatic hydrocarbons(PAHs),were well separated on the SiO2@COF packed column.In addition,the SiO2@COFETTA-Td stationary phase was successfully applied in the separation of phthalates,antiepileptic drugs,and polycyclic aromatic hydrocarbons(PAHs)in PM2.5,further demonstrating its potential for the separation and analysis of complex biological samples.Through this experiment,students can master the operation of HPLC,deepen and consolidate their understanding of chromatography,grasp the principles of core-shell stationary phase separation,and improve their scientific thinking and innovation abilities.
High performance liquid chromatographyCovalent organic frameworksCore-shell stationary phaseInstrumental analysis experiment
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