首页|澳洲茄碱通过调控Bcl-2/Bax/caspase-3信号通路促进非小细胞肺癌发生凋亡

澳洲茄碱通过调控Bcl-2/Bax/caspase-3信号通路促进非小细胞肺癌发生凋亡

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目的 探讨龙葵活性成分澳洲茄碱对非小细胞肺癌细胞PC9增殖、凋亡的影响。方法 体外培养PC9细胞，设对照组(0 μmol/L)及澳洲茄碱不同剂量组(0、2、5、10、15、20、25 μmol/L)，CCK-8试剂盒检测澳洲茄碱对PC9细胞的增殖抑制作用;TMRE检测线粒膜电位;caspase3/7活性试剂盒联合GreenNuc™ Caspase-3/Annexin V-mCherry染色检测caspase-3活性;Annexin V-FITC/PI双染法检测细胞凋亡率;给药处理或者使用PTEN抑制剂后，Western blot检测细胞中相关蛋白的表达量。结果 与对照组相比，经澳洲茄碱干预24、48、72 h后，PC9细胞的活力均明显降低(P<0。05);经澳洲茄碱干预24 h后，细胞线粒体膜电位明显降低，而细胞凋亡比例明显升高(P<0。05);caspase-3/7活力、活细胞Caspase-3活性及cleaved caspase-3蛋白表达均显著升高(P<0。01);PI3K和Akt磷酸化水平降低(P<0。05);而PTEN、Bax蛋白表达上调(P<0。05);抗凋亡蛋白Bcl-2蛋白的表达下调(P<0。05)。结论 澳洲茄碱可通过调控Bcl-2/Bax/caspase-3通路及其上游蛋白活性而抑制PC9细胞增殖，促进其凋亡。
Solasonine promotes apoptosis of non-small cell lung cancer cells by regulating the Bcl-2/Bax/caspase-3 pathway
Objective To investigate the effect of solasonine,an active component of Solanum nigrum,on proliferation and apoptosis of non-small cell lung cancer PC9 cells.Methods PC9 cells were treated with 2,5,10,15,20,or 25 μmol/L solasonine,and the changes in cell proliferation were examined using CCK-8 assay.Tetramethyl rhodamine ethyl ester(TMRE)was used to detect the changes in mitochondrial membrane potential,and caspase-3/7 detection kit and GreenNuc™ caspase-3/Annexin V-mCherry kit for live cell were used to analyze the changes in caspase-3 of the cells.Annexin V-FITC/PI double staining was employed to analyze the apoptosis rate of the cells.The effect of PTEN inhibitors on solasonine-induced cell apoptosis was examined by detecting apoptosis-related protein expressions using Western blotting.Results Solasonine treatment for 24,48,and 72 h significantly lowered the viability of PC9 cells.The cells treated with solasonine for 24 h showed significantly decreased mitochondrial membrane potential and increased cell apoptosis with enhanced caspase-3/7 and caspase-3 activities and expression of cleaved caspase-3.Solasonine treatment significantly decreased phosphorylation levels of PI3K and Akt,increased the protein expressions of PTEN and Bax,and lowered the expression of Bcl-2 protein in the cells.Conclusion Solasonine inhibits proliferation and induces apoptosis of PC9 cells by regulating the Bcl-2/Bax/caspase-3 pathway and its upstream proteins.

solasoninelung cancerBcl-2/Bax/caspase-3 signaling pathwayhomologous phosphatase tensin proteinapoptosis

陈桂玲、廖晓凤、孙鹏涛、岑欢、舒盛春、李碧晶、黎金华

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广州中医药大学第二附属医院,广东 广州 510120

广东省第二人民医院,广东 广州 510317

南方医科大学生物医学工程学院,广东 广州 510515

澳洲茄碱 肺癌 Bcl-2/Bax/caspase-3通路 同源性磷酸酶-张力蛋白 细胞凋亡

国家自然科学基金青年项目中国博士后基金资助项目广州市科技计划项目广东省中医药局中医药科研项目

821044562020M67274520210202020520244026

2024

10.12122/j.issn.1673-4254.2024.06.11

南方医科大学学报
南方医科大学

南方医科大学学报

CSTPCD北大核心
影响因子：1.654
ISSN：1673-4254
年,卷(期)：2024.44(6)
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