Danshen Injection inhibits peritoneal dialysis fluid-induced endothelial-mesenchymal transition in HMrSV5 cells by regulating the TGF-β/Smad signaling pathway
Objective To investigate the inhibitory effect of Danshen Injection on endothelial-mesenchymal transition(EndMT)induced by peritoneal dialysis fluid in HMrSV5 cells and the role of the TGF-β/Smad signaling pathway in mediating this effect.Methods HMrSV5 cells cultured in 40％peritoneal dialysis solution for 72 h to induce EndMT were treated with 0.05％,0.1％and 0.5％Danshen Injection.CCK-8 assay was used to assess the changes in viability of the treated cells,and the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),transforming growth factor-β(TGF-β),and vascular endothelial growth factor(VEGF)in the cell supernatant were detected using ELISA;Western blotting was performed to detect the protein expressions of E-cadherin,α-smooth muscle actin(α-SMA),p-Smad 2/3,and Smad 7 in the cells.Results Culture in 40％peritoneal dialysis fluid for 72 induced significant EndMT in HMrSV5 cells,which exhibited obviously lowered cell viability.Danshen Injection within the concentration range of 0.025％-1.5％did not significantly affect the viability of the cells.Exposure of HMrSV5 cells to peritoneal dialysis fluid for 72 h significantly increased the production of IL-6,TNF-α,TGF-β and VEGF,upregulated the protein expressions of α-SMA and p-Smad 2/3,and lowered the expressions of E-cadherin and Smad7 proteins.Treatment of the exposed cells with Danshen injection significantly increased cell viability and cellular expressions of E-cadherin and Smad 7 proteins and reduced the production of IL-6,TNF-α,TGF-β and VEGF and the protein expressions of α-SMA and p-Smad 2/3.Conclusion Danshen Injection can suppress peritoneal dialysis fluid-induced EndMT in HMrSV5 cells possibly by regulating the TGF-β/Smad signaling pathway.
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