Construction and Bioimaging of PPi Fluorescent Probes Based on Iron Complexes
A novel ligand ARC was synthesized by condensation reaction of naphthalene[2,1-b]furan-2-carbohy-drazide and 4-formyl-3-hydroxy-N-butyl-1,8-naphthalenediimide.The ligand ARC was synthesized by 1∶1 complex reaction with Fe3+.It was studied by high-resolution mass spectroscopy(HR-MS),NMR hydrogen spectroscopy(1H NMR),UV spectroscopy,and fluorescence spectroscopy.The results showed that the probe ARC-Fe3+can identify pyrophosphate with high selectivity and sensitivity through fluorescence"OFF-ON".The detection limit of the probe ARC-Fe3+to identify pyrophosphate is 1.12×10-8 mol/L,and the response to pyrophosphate can be completed within 60 s.The probe ARC-Fe3+has been successfully applied to the fluorescence imaging of pyrophosphate in cells and living mice,so it can be applied to various fields such as food,medicine,medical treatment and environment in the future.
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