Optimization of Tissue Culture and Rapid Propagation Technology of Xianghai No.1 Mulberry
In order to improve the efficiency of mulberry seedling raising,the stem segment of Xianghai No.1 Mul-berry was used as explants to optimize its tissue culture and rapid propagation system through axillary bud induction culture,proliferation culture and rooting culture.The results showed as follows:the optimal medium for axillary bud induction culture was MS+6-BA3.0 mg·L-1+IAA0.1 mg·L-1,and the induction rate was 78.3％;the op-timal medium for proliferation culture was 4/5MS(large amount)+6-BA0.1 mg·L-1+NAA0.15mg·L-1,and the average multiplication ratio was 5.12 in 30 days;the optimal medium for rooting culture was 1/2MS(large a-mount)+1/4MS(ferritic salt)+IBA1.0 mg·L-1,and the rooting rate reached about 82.5％.Rooting began 8 days later,and the seedlings could be transplanted and cultivated 28 days later.
万方数据
维普
