The enzyme inhibition rate rapid detection method used for the detection of pesticide residues in traditional Chinese medicinal materials
The method of enzyme inhibition rate is adopted to rapid detect the residues of organophorus and carbamate pesti-cides in traditional Chinese medicine,thus establishing a rapid detection method for pesticide residues traditional Chinese medicine.Regarding enzyme inhibition rate as the index of investigating,the effects of the extraction time,sample volume,and inhibition time on the detection rate were separately investigated.The optimal detection conditions for organophospho-rus and carbamate pesticides in traditional Chinese medicine were determined as follows:the extraction time was 10min,the sample volume of the target components was 1.5mL,the time of inhibitory was 15 minutes.The conducting recovery experi-ments by adding carbofuran,dichlorvos and monocrotophos to pesticide-free traditional Chinese medicinal materials(in-cluding Cardamom,Goldthread,Stone Olive,Dendrobium officinale and Lily),the average recovery rates is in 81.8-109.0%,with relative standard deviations of less than 10%.The detection method limit is 0.02-1 mg/kg for pesticides such as carbofuran,dichlorvos and monocrotophos.The results of the sample analyzed using both rapid testing and traditional chromatographic methods show good consistency.
traditional Chinese medicinalenzyme inhibition ratepesticide residuesrapid detection
万方数据
维普
