Pre-column Derivatization GC Method of Chiral Diols
A method of the chiral separation of 1,3-butanediol,2,5-hexadiol,3,6-octanediol and 3,6-octanediol enantio-mers via derivatization-capillary column gas chromatography was established and validated.The diol enantiomers were de-rivatized with N-methyl-n-(trimethylsilane)trifluoroacetamide or trifluoroacetic anhydride and then were separated by CP-Chirasil-Dex CB column,split sampling,and FID as detector.The linearity of the methods were proven well with the correlation coefficients were 0.9994,0.9997,0.9990,and 0.9971,as well as the detection ranges of 2.50-8×103 µg/mL,1.30-8×103µg/mL,3.5-1×104µg/mL and 5-1×104µg/mL.All the resolution factors of enantiomers separation were above 2.The detection limits of 4 analytes were 0.8µg/mL,0.4µg/mL,1.8µg/mL,and 2.5µg/mL,respectively.The method is ap-plied in the determination of(R)-1,3-butanediol,(2R,3R)-2,3-butanediol,(2S,5S)-2,5-hexanediol and(3S,6S)-3,6-octanediol which were produced by enzymatic reaction and the enzymatic reaction monitoring as well.
万方数据
维普
