Determination of Etomidate in Blood Samples by UPLC-MS/MS
In this study,a method for the determination of etomidate in blood samples by UPLC-MS/MS was established.The whole blood were extracted by acetonitrile,then etomidate was determined with the UPLC-MS/MS system coupled with ESI+mode and multiple reaction monitoring(MRM).Etomidate gave linear calibration curves in the range of 0.25-20 ng/mL with correlation coefficients(R2)better than 0.999.The detection limit(LOD)of etomidate was 0.05 ng/mL and the quantification limit(LOQ)was 0.25 ng/mL.At spiked levels of 0.25、1.0、10 ng/mL,the recoveries were in the range of 93.7％-98.5％with relative standard deviations(RSDs)less than 6.3％.The method is simple,rapid,sensitive and accu-rate for the determination of trace etomidate in whole blood samples.
万方数据
维普
