Extraction,structural characterization and bioactivity of polysaccharides from Gongshisong
Gongshisong polysaccharide was obtained from Gongshisong by water extract-alcohol precipitation method.The structure of Gongshisong polysaccharide was characterized by high performance gel permeation chromatography,pre-col-umn derivatization HPLC,ultraviolet and infrared spectroscopy,and Congo red assay,etc.The antioxidant activity of Gong-shisong polysaccharide was evaluated by antioxidant assay,and the inhibitory effect of Gongshisong polysaccharide on hepa-tocellular carcinoma cells HepG2 and hepatocellular cells LO2 was preliminarily investigated by MTT assay.The results showed that the relative molecular weight of Gongshisong polysaccharide was distributed from 50,325 to 115,144 Da,and consisted of mannose,glucose,galactose and arabinose with triple-helical structure.In vitro antioxidant activity assay showed that Gongshisong polysaccharide exhibited strong scavenging ability against DPPH,ABTS,and hydroxyl radicals.Anti-tumor assay showed that Gongshisong polysaccharide has the ability to inhibit the proliferation of HepG2.
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