Screening of efficient benzene-degrading microbial consortia and degradation performance
A benzene-degrading microbial consortia was obtained from the enrichment and domestication of contaminated soil.Its degradation characteristics were explored and degradation conditions were optimized.Benzene,toluene,ethylbenzene and xylene were used as carbon sources for multi-cycle domestication.Com-munity structure analysis was carried out by high-throughput sequencing technology,the degradation effect of microbial consortia on benzene was detected by gas chromatography,and the degradation conditions were opti-mized by changing a single factor.The BTEX-degradation microbial consortia after domestication were mainly Pseudomonas,accounting for 56.07%.The optimum conditions for benzene degradation by microbial consortia were an an initial pH of 7,a temperature of 30℃and benzene concentration of 200 mg/L,and the degrada-tion rate was more than 80%.The addition of Fe3+and Mn2+ions could enhance the benzene degradation effi-ciency,and the degradation of benzene by 0.5 mg/L Mn2+and 2.0 mg/L Fe3+reached 90.97%and 86.63%,respectively,which were 8.57%and 4.23%higher than before addition.The degradation process of benzene by the microbial consortia conformed to the first-order kinetic equation,and the half-life of the microbial con-sortia to degrade 200 mg/L benzene was only 12.27 h.This microbial consortia has a strong ability to degrade benzene,and has a certain application prospect for the remediation of benzene contaminated sites.
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