Study on Formula Optimization of Mother Culture Medium of Sanghuangporus
[Objective]To screen the best mother medium of Sanghuang for cultivating high-quality Sanghuangporus.[Method]Sanghuangporus strain was inoculated into the medium with different formulas for preliminary screening and re-screening.Through morphological comparison,the optimal mother culture medium formula was selected from the aspects of hypha growth vigor,growth rate and others.[Result]The results of preliminary screening showed that sucrose and glucose were selected as the best carbon sources with fast growth,not easy to aging,no yellow water,and dense growth.Peptone and tryptone were selected as the best nitrogen source with fast growth,not easy to aging,good growth vigor and no yellow water.The results of re-screening showed that the hypha had good growth vigor,dense and fast growth when formula of 1/4 glucose+3/4 sucrose was used.[Conclusion]The formula of 1/4 glucose+3/4 sucrose is recommended in production,containing 200 g potato,5 g glucose,15 g sucrose,20 g agar and 1 000 mL water.
万方数据
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