Tissue Culture for Rapid Propagation of Dracaena cambodiana
[Objective]The objective of this study is to explore the effects of different disinfection methods and hormone ratios on rapid propagation technology of Dracaena cambodiana tissue culture,and to provide references for establishing a rapid propagation system of D.cambodiana tissue culture.[Method]The effects of different sterilization times,plant hormone ratios and concentrations on the induction,proliferation,and rooting of adventitious buds were explored using'Space'and'68#'as materials.[Result]The results showed that the aseptic materials could be obtained using the stem with apical bud when it was disinfected by 75%alcohol for 20 seconds and 0.1%mercuric chloride for 8 minutes,achieving the best disinfection effect.The optimal medium for inducing adventitious buds was MS+5.0 mg/L 6-BA+3.0 g/L Huabao;the optimal proliferation medium was MS+2.0 mg/L 6-BA+0.1 mg/L NAA,and the proliferation coefficients of'Space'and'68#'reached 5.35 and 4.87,respectively;the optimal rooting medium was MS+1.0 mg/L NAA and the rooting rates of'Space'and'68#'were both above 99%,with rooting numbers of 9.2 and 11.2,respectively.[Conclusion]The establishment of tissue culture and rapid propagation system of D.cambodiana with apical bud stem as explants will lay a good foundation for the industrial production and genetic transformation of D.cambodiana.
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