In Vitro Culture and Plant Regeneration of Internode Stem Segment of Jasminum sambac
[Objective]To obtain regenerated plants by in vitro culture of internode stem segment of Jasminum sambac and fill the gap in reports on the regenerated plants obtained from callus.[Method]The internode stem segments of J.sambac were selected as the explants and different concentrations of 84 disinfectant and Tween-20 were selected for suitable explant disinfection method.On this basis,9 combination of different concentration of 6-BA and NAA were set to screen suitabl media for callus induction,differentiation and complete plant regeneration.[Result]When 0.1 mL Tween-20 was added to 20%84 disinfectant and internode stem segments were disinfected for 12 min,the contamination rate of the explants was low and the survival rate was high.The callus induction rate reached 100%on MS medium+1.5 mg/L 6-BA+1.5 mg/L NAA+30 g/L sucrose+6 g/L Agar.After 2 months of growth on this medium,the callus generated from stem segments could differentiate into shoots and roots.When the callus continued to grow on this medium at the 6th month,stems and leaves began to appear.When the callus continued to grow to the 8th month,the complete plants began to appear.When cultivating to the 12th month,the callus began to browning gradually,the growth of the plant slowed down,and some leaves appeared scorched.[Conclusion]The internode stem callus could be regenerated into complete plants without secondary culture,which provided a way to obtain transgenic plants through genetic transformation of Jasminum sambac.
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