茉莉花节间茎段的离体培养和植株再生
In Vitro Culture and Plant Regeneration of Internode Stem Segment of Jasminum sambac
胡莉 1张蕖 2胡双玲 2尹秦婠 2袁媛2
作者信息
- 1. 福建农林大学园艺学院,福建福州 350002;四川化工职业技术学院,四川泸州 646300
- 2. 福建农林大学园艺学院,福建福州 350002
- 折叠
摘要
[目的]通过双瓣茉莉(Jasminum sambac)节间茎段离体培养获得再生植株,填补通过愈伤组织获得再生植株报道的空白.[方法]以双瓣茉莉节间茎段为外植体,设置不同 84 消毒液和Tween-20浓度组合筛选适宜的外植体消毒方式,并在此基础上设置 9 种不同的 6-BA和NAA浓度组合筛选适宜的培养基进行愈伤组织诱导、分化及完整植株再生.[结果]茉莉花节间茎段经20%的84消毒液中滴加0.1 mL Tween-20 消毒 12 min时,污染率低且成活率高;在MS+1.5 mg/L 6-BA+1.5 mg/L NAA+30 g/L 蔗糖+6 g/L 琼脂培养基上愈伤组织诱导率达 100%.在此培养基生长 2 个月后,茎段产生的愈伤组织开始分化形成芽和根;培养至第 6 个月,开始形成茎和叶;培养至第 8 个月,开始形成完整植株;培养至第 12 个月时,愈伤组织开始逐渐褐化,植株的生长放缓,部分叶片出现枯焦.[结论]茉莉花节间茎段诱导愈伤组织可在不继代的情况下再生为完整植株,为茉莉花遗传转化获得转基因植株提供了途径.
Abstract
[Objective]To obtain regenerated plants by in vitro culture of internode stem segment of Jasminum sambac and fill the gap in reports on the regenerated plants obtained from callus.[Method]The internode stem segments of J.sambac were selected as the explants and different concentrations of 84 disinfectant and Tween-20 were selected for suitable explant disinfection method.On this basis,9 combination of different concentration of 6-BA and NAA were set to screen suitabl media for callus induction,differentiation and complete plant regeneration.[Result]When 0.1 mL Tween-20 was added to 20%84 disinfectant and internode stem segments were disinfected for 12 min,the contamination rate of the explants was low and the survival rate was high.The callus induction rate reached 100%on MS medium+1.5 mg/L 6-BA+1.5 mg/L NAA+30 g/L sucrose+6 g/L Agar.After 2 months of growth on this medium,the callus generated from stem segments could differentiate into shoots and roots.When the callus continued to grow on this medium at the 6th month,stems and leaves began to appear.When the callus continued to grow to the 8th month,the complete plants began to appear.When cultivating to the 12th month,the callus began to browning gradually,the growth of the plant slowed down,and some leaves appeared scorched.[Conclusion]The internode stem callus could be regenerated into complete plants without secondary culture,which provided a way to obtain transgenic plants through genetic transformation of Jasminum sambac.
关键词
茉莉花/茎段/愈伤组织/组培/再生/完整植株Key words
Jasminum sambac/Stem/Callus/Tissue culture/Regeneration/Complete plant引用本文复制引用
基金项目
福建省自然科学基金项目(2022J01588)
福建农林大学科技创新专项基金(KFB23033)
出版年
2024
国家科技期刊平台
NSTL
万方数据