Study on Selection and Application of Culture Medium of Particle Pre-culture Spawn of Stropharia rugosoannulata
[Objective]The aim is to screen suitable spawn culture media for Stropharia rugosoannulata and develop particle pre-culture spawn with strong mycelial vitality,numerous germination points,and loose uniformity.The appropriate inoculation amount of spawn using vent plastic bag was established.[Method]Using'S.rugosoannulata No.8'as the experimental strain and the mycelial growth rate as the evaluation index,a single factor experiment was conducted to compare the effects of particle spawn propagation and cultivation on different media containing main components such as millet,fine sawdust,corn cob,and rapeseed meal,and compare the effects of different inoculation amount on the bag-filled time of spawn using vent plastic bag.[Result]The optimal formula for the particle pre-culture spawn medium of S.rugosoannulata was determined to be XGM,with 50%millet,2%sucrose,20%fine sawdust,26%diatomaceous earth,and 2%light calcium carbonate.The carbon-to-nitrogen ratio in the medium was 32.5,and the growth rate of the mycelia of the particle pre-culture spawn was 2.99 mm/d.The optimal inoculation amount was 1.25%and the average bag-filled time was 13.4 days using particle pre-culture spawn XGM to expand spawn in vent plastic bag.[Conclusion]The culture medium of particle pre-culture spawn was optimized and the production process of S.rugosoannulatain using vent plastic bag was preliminarily established,which laid a foundation for industrial production of S.rugosoannulata spawn.
StrophariarugosoannulataParticle pre-culture spawnSpawn using vent plastic bagApplication
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