Screening of Liquid Fermentation Medium and culture condition for Dictyophora rubrovolvata
[Objective]Screen the liquid spawn fermentation medium for Dictyophora rubrovolvata and provide references for the application of liquid spawn of D.rubrovolvata in industrialized cultivation.[Method]The effects of medium nutrients and culture methods on liquid fermentation of D.rubrovolvata were investigated with the mycelium biomass as the indicator.[Result]Maltose syrup,corn syrup,corn flour,soybean powder for fermentation and soybean flour are suitable for the liquid fermentation of D.rubrovolvata.When the amount of corn syrup was 20 g/L,the mycelial biomass was 7.13 g/L.Corn flour,soybean powder for fermentation and soybean flour could significantly promote the mycelial biomass in the test range,when the usage was 4 g/L,the mycelial biomass reach the maximum,which were 2.1 g/L,1.8 g/L,and 1.7 g/L respectively.[Conclusion]Using 250 mL Erlenmeyer flasks,the suitable culture conditions for D.rubrovolvata were listed as follows:initial medium pH 5.5,liquid volume 100-120 mL,inoculation volume 16%,and the terminal point of liquid spawn is 14 d.
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