Isolation and Identification of the Pathogen Causing Kiwifruit Root Rot and the Resistance Analysis of Different Germplasms to the Pathogen
[Objective]The type of pathogen causing the root rot of kiwifruits in Mingxi County,Sanming City,Fujian Province need to be clarified.And the differences in resistance to the pathogen among different kiwifruit germplasms also need to be studied.[Method]The pathogen was isolated by using tissue separation method.The morphological observation and molecular biological methods were used to identify the pathogen.Then,its pathogenicity was tested according to Koch's postulates.And the resistance to the pathogen among different kiwifruit germplasms was analyzed.[Result]A pathogen(named 0814-1)was isolated from typical root rot tissues of kiwifruits.The colony was white on the surface,and white or orange on the back,with an irregular margin.The ITS an TEF were cloned from 0814-1 and sequenced,and the blast analysis in NCBI database showed that they shared more than 99%identity with that of Fusarium concentricum.The phylogenetic analysis also revealed that 0814-1 clustered with F.concentricum in the same branch.Pathogenicity test results showed that the symptoms caused by 0814-1 were consistent with that in the field,fulfilling Koch's postulates.Based on the results of morphology,molecular biology,and Koch's postulates,the isolated pathogen was identified as F.concentricum.Resistance analysis showed that there were significant differences in resistance to the pathogen 0814-1 among those germplasms,and'Jinkui'and'White'were screened as disease-resistant germplasms.[Conclusion]A pathogen causing kiwifruit root rot was isolated and identified as F.concentricum,then two disease-resistant germplasms were screened.Those results laid the foundation for the integrated control of kiwifruit root rot and resistance breeding.
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