摘要
[目的]为探究不同花色品种山茶花代谢产物的积累和关键基因表达特征,并解析二者间的相互关系.[方法]以6种不同花色性状的山茶花花瓣为材料,测定其花色苷、总酚、类黄酮和原花青素含量,采用实时荧光定量PCR检测产物合成相关基因的表达水平,并构建产物积累和基因表达的网络作用关系图.[结果]山茶花深色品种花色苷积累水平高于浅色和白色品种,紫红色品种'午夜'的花色苷、类黄酮和原花青素含量显著高于其他品种,分别达到572.18 µg/g、18.93 mg/g和97.29 mg/g;深色和浅色品种总酚含量高于中度着色的品种,红色品种'赤丹'的含量最高,达到103.13 mg/g,'午夜'也具有较高的积累水平;定量表达分析显示,不同品种的相关基因表达水平具有特异性,'午夜'呈现上游基因(CHS和F3H)高表达,下游基因(F3'H、DFR、ANS和UFGT)低表达特征;相关性分析显示,类黄酮和原花青素积累与F3H、ANS、UFGT的表达呈正相关,花色苷的积累与CHS、CHI、F3'H呈正相关,而与F3H、DFR、ANS呈负相关.[结论]山茶花品种'午夜'具有较高花色苷、总酚、类黄酮和原花青素积累水平,合成通路上、下游基因对花色苷合成积累分别具有促进和抑制作用,而下游基因总体促进类黄酮和原花青素的积累.
Abstract
[Objective]This study aims to investigate the accumulation of metabolites and the expression characteristics of key genes in camellia(C.)japonica flowers with varying flower colors,as well as to analyze the relationship between the two factors.[Method]Six different color traits of C.japonica petals were selected as materials to determine the contents of anthocyanins,total phenols,flavonoids,and proanthocyanidins.Real-time fluorescence quantitative PCR was employed to assess the expression levels of genes associated with product synthesis,and a network diagram illustrating the relationship between metabolite accumulation and gene expression was constructed.[Result]The accumulation of anthocyanins in dark colored cultivars of C.japonica were found to be higher than that in light and white cultivars.Notably,the purple-red cultivar'Midnight'exhibited significantly higher contents of anthocyanins,flavonoids,and proanthocyanidins,reaching 572.18 µg/g,18.93 mg/g,and 97.29 mg/g,respectively.Furthermore,the total phenolic content in dark and light colored cultivars exceeded that of moderately colored cultivars,with the red cultivar'Chidan'displaying the highest content at 103.13 mg/g,while'Midnight'also showed a relative high level of accumulation.Quantitative expression analysis revealed that gene expression levels varied specifically among different cultivars.'Midnight'demonstrated high expression of upstream genes(CHS and F3H)and low expression of downstream genes(F3'H,DFR,ANS,and UFGT).Correlation analysis indicated that flavonoid and proanthocyanidin accumulation was positively correlated with the expression of F3H,ANS,and UFGT.Additionally,anthocyanin accumulation was positively correlated with the expression of CHS,CHI,and F3'H,and negatively correlated with F3H,DFR,and ANS.[Conclusion]The C.japonica'Midnight'exhibits a high accumulation of anthocyanins,total phenols,flavonoids,and proanthocyanidins.The upstream and downstream genes in the synthesis pathway have both promoting and inhibitory effects on anthocyanin synthesis and accumulation,respectively,while downstream genes generally promote the accumulation of flavonoids and proanthocyanidins.
维普
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