A Study on the Tissue Culture Spathiphyllum floribundu
Using the MS culture medium,the shoot tip of Spathiphyllum floribundu was induced and cultured with the dif ferent hormone in different concentration levels.The results revealed that the suitable culture medium for shoot tip induction was the improved MS+6BA 1.5～3. 0 mg/L+ NAA 0.5～1.0 mg/L,whose induction rate reached 90%; the suitable cultur e medium for adventitious bud multiplication was the improved MS+6BA 0.5～2.5 m g/L+NAA 0.5～1.5 mg/L,in which the annual multiplication coefficient of adventi tious bud gained 4.212;the suitable culture for rooting induction was 1/2 MS+NA A 1.0 mg/L or 1/2MS+IBA 1.0 mg/L,whose rooting rate was more than 90%. The fi rst rate generation-continuing seedling were selected,transplanted and cultured for taking root on the culture medium outside the test-tube,whose survival rat e reached more than 85%.So,it was an effective way to quicken the factory seedl ing speed and to reduce the cost of tissue culture seedling.
国家科技期刊平台
NSTL
万方数据
维普
