Study on somatic embryogenesis of elite longleaf pine families
Using the megagametophyte including immature zygotic embryos from the different cone collection time of the elite longleaf pine families 20 - 820,20 - 803,20 - 822,20 - 805,20 - 825 as explants, combining the 4 patent media AL, TX, LP, DS for somatic embryogenesis with different hormones and its levels or without hormone to form 16 initiation media, the effects of different patent media, families, cone collection time on initiation rate were studied. The results showed that the most suitable collection date of cone for somatic embryogenesis is July 16 and July 24 when immature zygotic embryos is at development stage 1,2. There is not significant difference in embryogenic suspensor mass initiation rate among 5 families, but initiation rate of different basic media, different hormone combinations have significant difference. The combination of LP basic medium with TX hormone is most suitable for initiation of somatic embryos of longleaf pine, in which initiation rates 28%.
Longleaf pinesomatic embryogenesispatent mediainitiation rateembryogenic suspensor mass
国家科技期刊平台
NSTL
万方数据
维普
