首页|‘闽48’和‘闽43’油茶自交与异交花粉管的荧光显微观察

‘闽48’和‘闽43’油茶自交与异交花粉管的荧光显微观察

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
以‘闽48’、‘闽43’为材料,结合人工控制授粉和花粉管荧光显微观察,对比授粉后自交和异交花粉管生长的差异.结果显示,自交和异交花粉均能在柱头上萌发,但自交和异交花粉管在各时间段生长速度存在差异.异交花粉管生长总体呈快-慢-快趋势,分别在授粉后6-7h及24-30 h出现峰值,生长速率最高达67 μm· h-1,并于授粉后76 h穿过花柱基部;自交花粉管在花柱中则呈现波动生长:授粉后6h出现停滞期,9h恢复生长,24 h生长速率急剧上升,30-48 h达到最高,为32.22 μm· h-1,最终于授粉后127 h停止生长,此时花粉管出现大量胼胝质堵塞,无法穿过基部.
A fluorescence microscope observation on self-pollination and cross-pollination of pollen tubes in Camellia oleifera ‘Min 48' and ‘Min 43'
Using ‘Min 48',‘Min 43' as materials,control pollination with fluorescence microscopy observation was combined to investigate pollen tubes growth differences between self-pollination and cross-pollination after pollination.The results showed that pollens by self-pollination and cross-pollination germinated normally,while pollen tubes growth rates were different in different period.The cross-pollination growth rate showed an ‘ up-down-up' tendency,appearing 2 extreme value (up to 67 μm· h-1) in the period of 6-7 h and 24-30 h separately.Then the pollen tubes penetrated style base in 76 h after pollination.Comparing with cross-pollination,self-pollination presented a wave growth tendency:lag phase occurred in 6 h after pollination,then recovered growth in 9 h after pollination.A step rise happened in 24 h after pollination.Self-pollination pollen tubes kept a rapid growth tendency until they reached the peak value (32.22 μm· h-1) in the period of 30-48 h.In the final,the pollen tubes stopped growing and couldn't penetrate style base for callose blockage in pollen tube tip in 127 h after pollination.

Camellia oleifera Abel.pollen tubefluorescence microscopyself-pollinationcross-pollination

郑碧娟、陈世品、陈辉、何艺凡、林文俊

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福建农林大学林学院,福建福州350002

油茶 花粉管 荧光显微镜技术 自交 异交

高等学校博士学科点专项科研项目科技部科技人员服务企业行动项目福建省科技厅高校产学重大专项福建省科技厅平台建设项目福建农林大学林学院青年基金

K4112020A2009GJC400062010N50062010N20016112c039p

2014

森林与环境学报
福建农林大学

森林与环境学报

CSTPCDCSCD北大核心
影响因子：0.964
ISSN：2096-0018
年,卷(期)：2014.34(1)
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