Variance analysis of protein extraction and expression for Chinese fir needles under low phosphorus stress
In order to obtain the protein profile with high repeatability and resolution under the condition of phosphorus deficiency,the high-speed centrifugation of TCA-acetone precipitation and the optimized electrophoresis including the extraction of whole-cell protein in Chinese fir needles,the protein cleavage and the condition of electrophoresis were used in this study.The protein extraction of fir needles under 5 days low-phosphorus treatment was conducted,and the comparison of protein profile between control and the treatment samples were made by using Image Master 2D Platinum 7 software.The results showed that there were 59 protein points of low phosphorus treatment significantly different from the control sample,which included 52 upregulated and 7 downregulated points.
Chinese firneedlesproteinlow-phosphorus stresstwo-dimensional electrophoresis
国家科技期刊平台
NSTL
万方数据
维普
