首页|福建杉木王种质资源的遗传多样性

福建杉木王种质资源的遗传多样性

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为研究福建省杉木王种质资源的遗传多样性,在全面调查的基础上收集了福建省44份杉木王种质,并采用简单序列重复区间扩增多态性( ISSR)分子标记的方法分析其群体遗传多样性。结果表明,福建省杉木王主要分布在闽西、闽南和闽北地区,闽东地区分布较少。29条ISSR引物共扩增出237条带,平均每条引物扩增8.17条带;共有212条带具有多态性,多态性条带比率89%。福建省杉木王群体Nei′s基因多样性指数为0.3428, Shannon信息指数为0.5026,显示出较高的遗传多样性。非加权类平均法聚类分析和遗传结构分析均显示,福建杉木王可划分为5个类群,其类群划分结果与样本间的地理位置分布较为一致。
Genetic diversity of germplasm resources of the king of Chinese fir in Fujian provenances
On the basis of comprehensive survey, 44 samples of the king of Chinese fir germplasm in Fujian provenances were collected, and the population genetic diversity was detected by inter-simple sequence repeats ( ISSR) molecular markers. The results showed that the king of Chinese fir were mainly distributed in western, southern and northern of Fujian, but rarely distributed in eastern. Twenty-nine primers were amplified into 237 bands, 8.17 bands on average from a primer amplification. Two hundred and twelve bands were polymorphic, accounting for 89%. The population Nei′s genetic diversity index was 0. 342 8, and the Shannon information index was 0.502 6, which revealed a relatively high genetic diversity. Both of the unweighted pair-group method with arithmetic means ( UPGMA) clustering and genetic structure analysis showed the king of Chinese fir in Fujian provenances could be divided into 5 groups, which were comparatively agree with the geography distribution.

Chinese firgenetic diversityinter-simple sequence repeats markergermplasm resourcesgenetic structure

李明、黄木生、苏烁烁、陈细贞、马祥庆

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福建农林大学林学院,福建 福州350002

国家林业局杉木工程技术研究中心,福建 福州350002

南平市林业局,福建 南平353000

杉木 遗传多样性 ISSR标记 种质资源 遗传结构

国家自然科学基金福建农林大学林学院青年科学基金

U14052116112C035006

2016

森林与环境学报
福建农林大学

森林与环境学报

CSTPCDCSCD北大核心
影响因子:0.964
ISSN:2096-0018
年,卷(期):2016.36(3)
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