In order to uncover the possible allelopathy of Leucaena leucocephala (Lam.) de Wit on native species and its implication for forest management, the effects of aqueous extracts of fresh root and leaf litter on seed germination, seedling survival and seedling growth were tested using Cynodon dactylon Linn. and Medicago sativa Linn. as recipients. The results showed that both the aqueous extracts of L. leucocephala fresh root and leaf litter inhibited seed germination and seedling survival of the two species, and the inhibition increased with the concentration growing. Among of them, the fresh root extracts significantly inhibited seed germination and seedling survival of M. sativa, but not significantly on C. dactylon. The seed germination rate and seedling survival rate of M. sativa reduced by 21.06% and 28.59%, respectively, when adding the fresh root extracts at the concentration of 0.125 g·mL-1 , compared with the control group. The leaf litter extracts had significant inhibition on seed germination of both species and seedling survival of M. sativa, but no significant inhibition on seedling survival of C. dactylon. Comparing with the control group, the seed germination rate and seedling survival rate of M. sativa reduced by 30.14% and 15.71%, respectively, and seed germination rate of C. Dactylon, reduced 49.08% when adding the leaf litter extracts at a concentration of 0.125 g·mL-1 . The aqueous extracts of fresh root and leaf litter of L. leucocephala promoted seedling growth of two recipients and the effect decreased with the concentration increasing. The allelopathic effect of leaf litter aqueous extracts was stronger than that of fresh root aqueous extracts. The response of M. sativa to the allelopathy of L. leucocephala was more sensitive than that of C. dactylon.